Document Detail


5-Azacytidine (5Az) induces apoptosis in PC12 cells: a model for 5Az-induced apoptosis in developing neuronal cells.
MedLine Citation:
PMID:  9151133     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our previous in vivo and in vitro studies showed that 5-azacytidine (5Az), a cytidine analog, induced apoptosis in developing neuronal cells in mice. To develop a system in which the precise molecular mechanism of 5Az-induced apoptosis in developing neuronal cells could be elucidated, we carried out the present study with PC12 cells. These cells are derived from a rat pheochromocytoma and extrude neurites in response to nerve growth factor (NGF). Light microscopy showed dose-dependent pyknotic and karyorrhectic changes in undifferentiated PC12 cells. Although they were less sensitive to 5Az, NGF-treated differentiated cells showed the same changes. Analysis by the TUNEL method (an in situ method for the detection of apoptosis) showed positive signals in the pyknotic and fragmented nuclei of these cells. Transmission electron microscopy revealed margination, segmentation, and condensation of nuclear chromatin, cell body shrinkage, and cytoplasmic vacuolization. Scanning electron microscopy demonstrated bleb formation on the cell surface. These pathomorphological changes are typical of apoptosis. 5Az seemed to affect cells that were in the proliferative stage; when the cells were terminally differentiated, their sensitivity to 5Az appeared to decline. PC12 cells could be used as a pathomorphological and biochemical model for studies of 5Az-induced neuronal cell apoptosis at the molecular and genetic level.
Authors:
M M Hossain; H Nakayama; A Takashima; N Goto; K Doi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histology and histopathology     Volume:  12     ISSN:  0213-3911     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-07-17     Completed Date:  1997-07-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  439-45     Citation Subset:  IM    
Affiliation:
Department of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antimetabolites, Antineoplastic / pharmacology*
Apoptosis / drug effects*
Azacitidine / pharmacology*
Cell Differentiation / drug effects,  physiology
DNA Fragmentation
Microscopy, Electron
Microscopy, Electron, Scanning
Nerve Growth Factors / pharmacology
Neurons / drug effects*,  ultrastructure
PC12 Cells
Rats
Chemical
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 0/Nerve Growth Factors; 320-67-2/Azacitidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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