Document Detail


5-Aza-2'-deoxycytidine as inducer of differentiation and growth inhibition in mouse neuroblastoma cells.
MedLine Citation:
PMID:  2475229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the effect of 5-aza-2'-deoxycytidine (5-AZA-CdR) on the differentiation of murine 41A3 neuroblastoma cells. Neuroblastoma cells treated with 0.1-1.0 microM 5-AZA-CdR underwent differentiation; markers of neuronal functions, such as acetylcholinesterase activity and growth of nerve fibers, were expressed at a higher level in the drug-treated cells than in the controls. This increased expression was accompanied by significant hypomethylation of newly synthesized DNA. A secondary event seemed to be a partial inhibition of DNA synthesis, cell proliferation and colony-forming activity. These effects were more pronounced than those caused by the related cytidine analog, 1-beta-D-arabinosil-cytosine (ARA-C). The results obtained suggest that 5-AZA-CdR may be an effective agent for the growth control of human neuroblastoma cells.
Authors:
S Bartolucci; M Estenoz; A Longo; B Santoro; R L Momparler; M Rossi; G Augusti-Tocco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell differentiation and development : the official journal of the International Society of Developmental Biologists     Volume:  27     ISSN:  0922-3371     ISO Abbreviation:  Cell Differ. Dev.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-09-26     Completed Date:  1989-09-26     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  8811335     Medline TA:  Cell Differ Dev     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  47-55     Citation Subset:  IM    
Affiliation:
Dipartimento di Chimica Organica e Biologica, Università di Napoli, Italy.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / metabolism
Animals
Azacitidine / analogs & derivatives*,  pharmacology
Cell Differentiation / drug effects
Cell Division / drug effects
Cell Survival / drug effects
DNA / metabolism
Methylation
Mice
Neuroblastoma*
Tumor Cells, Cultured / cytology*,  drug effects,  metabolism
Chemical
Reg. No./Substance:
320-67-2/Azacitidine; 776B62CQ27/decitabine; 9007-49-2/DNA; EC 3.1.1.7/Acetylcholinesterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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