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5-Aminouracil treatment. A method for estimating G2.
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MedLine Citation:
PMID:  5551658     Owner:  NLM     Status:  MEDLINE    
Treatment of Vicia faba lateral roots with a range of concentrations of 5-aminouracil (5-AU) indicate that cells are stopped at a particular point in interphase. The timing of the fall in mitotic index suggests that cells are held at the S - G(2) transition. When cells are held at this point, treatments with 5-AU can be used to estimate the duration of G(2) + mitosis/2 of proliferating cells. Treatment with 5-AU can also be used to demonstrate the presence of subpopulations of dividing cells that differ in their G(2) duration. Using this method, 5-AU-induced inhibition, we have confirmed that in V. faba lateral roots there are two populations of dividing cells: (a) a fast-dividing population, which makes up approximately 85% of the proliferating cell population and has a G(2) + mitosis/2 duration of 3.3 hr, and (b) a slow-dividing population, which makes up approximately 15% of dividing cells and has a G(2) duration in excess of 12 hr. These estimates are similar to those obtained from percentage labeled mitosis (PLM) curves after incorporation of thymidine-(3)H.
S H Socher; D Davidson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  48     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1971 Feb 
Date Detail:
Created Date:  1971-05-30     Completed Date:  1971-05-30     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  248-52     Citation Subset:  IM    
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MeSH Terms
Mitosis / drug effects*
Plants / drug effects*,  metabolism
Thymidine / metabolism
Time Factors
Uracil Mustard / pharmacology*
Reg. No./Substance:
10028-17-8/Tritium; 50-89-5/Thymidine; 66-75-1/Uracil Mustard

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1971 by The Rockefeller University Press
Received Day: 26 Month: 5 Year: 1970
Revision Received Day: 11 Month: 8 Year: 1970
Print publication date: Day: 1 Month: 2 Year: 1971
Volume: 48 Issue: 2
First Page: 248 Last Page: 252
ID: 2108186
PubMed Id: 5551658

5-AMINOURACIL TREATMENT : A Method for Estimating G2
S. H. Socher
D. Davidson
From the Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106, and the Department of Biology, McMaster University, Hamilton, Ontario, Canada.
Dr. Socher's present address is the Department of Obstetrics and Gynecology, Vanderbilt University, Nashville, Tennessee 37203

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