Document Detail

5-Aminolaevulinic acid enhances ultrasound-induced mitochondrial damage in K562 cells.
MedLine Citation:
PMID:  20381823     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Ultrasound therapy is a new modality in the control of malignant cancers. The aim of the present study was to investigate the effect of 5-aminolaevulinic acid on the ultrasonic killing action in the cancer cells. MATERIALS/METHODS: The K562 cells as a cancer cell model were subjected to investigate the effect of 5-aminolaevulinic acid (5-ALA) on the ultrasonic killing action, in which the 5-ALA concentration was 2mM and the ultrasound exposure was 15 s at the intensity of 0.46 W/cm(2) and the frequency of 1.7MHz. Cytotoxicity was investigated 24h after ultrasound exposure using the trypan blue exclusion test. Ultrastructural cell morphology and mitochondrial changes were observed using transmission electron microscopy (TEM). Mitochondrial membrane potential (DeltaPsim) was evaluated using Rhodamine 123 assay. RESULTS: The death rates of the K562 cells in the controls including sham radiation and 5-ALA treatment alone were 1.81+/-0.13%, 1.27+/-0.20%, respectively. Those in ultrasound radiation alone and 5-ALA-ultrasound treatment were 12.61+/-2.63%, 46.87+/-4.09%, respectively. There were significant differences between 5-ALA-ultrasound treatment, ultrasound radiation alone and the controls (P<0.05). TEM showed that the mitochondria expanding and some vacuoles were found in the ultrasound-treated cells. After the treatment of ultrasound and 5-ALA together some cells presented typical characteristics of apoptotic cells, such as nuclear condensation and crescent formation. Mitochondria of the cells were damaged more seriously than those treated by ultrasound alone, there were obvious swollen mitochondria and mitochondria in which cristae were almost perfectly disappeared, and more vacuolar mitochondria were founded. Mitochondrial membrane potential (DeltaPsim) was more significantly collapsed when the K562 cells were exposed to 2mM 5-ALA for 4h and then 0.46 W/cm(2) irradiation of ultrasound than ultrasound radiation alone. CONCLUSION: 5-ALA pretreatment significantly enhanced the cytotoxicity of ultrasound radiation in K562 cells. The damage of mitochondria structure and function might be an important cause of cell death in K562 cells induced by the treatment of ultrasound radiation and 5-ALA together.
Yong He; Xinshu Xia; Chuanshan Xu; Heping Yu; Dingqun Bai; Junyan Xiang; Albert Wingnang Leung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-21
Journal Detail:
Title:  Ultrasonics     Volume:  50     ISSN:  1874-9968     ISO Abbreviation:  Ultrasonics     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-29     Completed Date:  2010-09-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0050452     Medline TA:  Ultrasonics     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  777-81     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier B.V. All rights reserved.
Department of Rehabilitation Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
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MeSH Terms
Aminolevulinic Acid / pharmacology*
Apoptosis / drug effects,  radiation effects
Cell Line, Tumor
K562 Cells / drug effects*,  radiation effects*
Membrane Potential, Mitochondrial
Mitochondria / drug effects*,  radiation effects*
Ultrasonic Therapy*
Reg. No./Substance:
106-60-5/Aminolevulinic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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