| 5-Aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside increases myocardial glucose uptake during reperfusion and induces late pre-conditioning: potential role of AMP-activated protein kinase. | |
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MedLine Citation:
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PMID: 19486332 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Late pre-conditioning protects against myocardial ischaemic-reperfusion injury. AMP-activated protein kinase (AMPK) is activated by exercise and 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR). Early pre-conditioning involves AMPK activation and increased myocardial glucose uptake. The aim of the present study was to determine whether AICAR activates myocardial AMPK and induces late pre-conditioning and whether myocardial glucose uptake during reperfusion was modulated. Twenty-four hours after AICAR treatment or exercise, Wistar rats were subjected to ischaemia and reperfusion in a Langendorff model and compared to control rats. AMPK activity increased immediately 2.5-fold in AICAR-treated animals (P < 0.01) and twofold in exercised animals (P < 0.05). AICAR and exercise reduced infarct size by 60% and 50% (both P < 0.01), respectively, and increased myocardial glucose uptake during reperfusion (AICAR; 45%, P < 0.05, exercise; 40%, P < 0.05). In conclusion, AICAR induces late pre-conditioning and increases myocardial glucose uptake during reperfusion in rat hearts. AICAR and exercise activate AMPK, suggesting a role of AMPK in the signalling mechanisms behind late pre-conditioning. |
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Authors:
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Steen B Kristiansen; Lasse Solskov; Niels Jessen; Bo Løfgren; Ole Schmitz; Jens Erik Nielsen-Kudsk; Torsten T Nielsen; Hans Erik Bøtker; Sten Lund |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-08 |
Journal Detail:
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Title: Basic & clinical pharmacology & toxicology Volume: 105 ISSN: 1742-7843 ISO Abbreviation: Basic Clin. Pharmacol. Toxicol. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-01 Completed Date: 2009-10-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101208422 Medline TA: Basic Clin Pharmacol Toxicol Country: Denmark |
Other Details:
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Languages: eng Pagination: 10-6 Citation Subset: IM |
Affiliation:
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Department of Cardiology, Aarhus University Hospital, Skejby Sygehus, Denmark. sbk@iekf.au.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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AMP-Activated Protein Kinases
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chemistry,
metabolism* Aminoimidazole Carboxamide / analogs & derivatives*, metabolism, pharmacology Animals Blood Glucose / analysis Glucose / metabolism* Glucose Transporter Type 4 / chemistry, metabolism Glycogen / analysis Heart / drug effects Ischemic Preconditioning, Myocardial* Male Myocardial Infarction / pathology Myocardial Reperfusion Injury / metabolism* Myocardium / chemistry, enzymology*, metabolism Physical Exertion / physiology Protective Agents / metabolism, pharmacology* Random Allocation Rats Rats, Wistar Ribonucleotides / metabolism, pharmacology* Ventricular Pressure / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Glucose Transporter Type 4; 0/Protective Agents; 0/Ribonucleotides; 3031-94-5/AICA ribonucleotide; 360-97-4/Aminoimidazole Carboxamide; 50-99-7/Glucose; 9005-79-2/Glycogen; EC 2.7.11.1/AMP-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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