Document Detail

5-Aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside increases myocardial glucose uptake during reperfusion and induces late pre-conditioning: potential role of AMP-activated protein kinase.
MedLine Citation:
PMID:  19486332     Owner:  NLM     Status:  MEDLINE    
Late pre-conditioning protects against myocardial ischaemic-reperfusion injury. AMP-activated protein kinase (AMPK) is activated by exercise and 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR). Early pre-conditioning involves AMPK activation and increased myocardial glucose uptake. The aim of the present study was to determine whether AICAR activates myocardial AMPK and induces late pre-conditioning and whether myocardial glucose uptake during reperfusion was modulated. Twenty-four hours after AICAR treatment or exercise, Wistar rats were subjected to ischaemia and reperfusion in a Langendorff model and compared to control rats. AMPK activity increased immediately 2.5-fold in AICAR-treated animals (P < 0.01) and twofold in exercised animals (P < 0.05). AICAR and exercise reduced infarct size by 60% and 50% (both P < 0.01), respectively, and increased myocardial glucose uptake during reperfusion (AICAR; 45%, P < 0.05, exercise; 40%, P < 0.05). In conclusion, AICAR induces late pre-conditioning and increases myocardial glucose uptake during reperfusion in rat hearts. AICAR and exercise activate AMPK, suggesting a role of AMPK in the signalling mechanisms behind late pre-conditioning.
Steen B Kristiansen; Lasse Solskov; Niels Jessen; Bo Løfgren; Ole Schmitz; Jens Erik Nielsen-Kudsk; Torsten T Nielsen; Hans Erik Bøtker; Sten Lund
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-08
Journal Detail:
Title:  Basic & clinical pharmacology & toxicology     Volume:  105     ISSN:  1742-7843     ISO Abbreviation:  Basic Clin. Pharmacol. Toxicol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-01     Completed Date:  2009-10-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101208422     Medline TA:  Basic Clin Pharmacol Toxicol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  10-6     Citation Subset:  IM    
Department of Cardiology, Aarhus University Hospital, Skejby Sygehus, Denmark.
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MeSH Terms
AMP-Activated Protein Kinases / chemistry,  metabolism*
Aminoimidazole Carboxamide / analogs & derivatives*,  metabolism,  pharmacology
Blood Glucose / analysis
Glucose / metabolism*
Glucose Transporter Type 4 / chemistry,  metabolism
Glycogen / analysis
Heart / drug effects
Ischemic Preconditioning, Myocardial*
Myocardial Infarction / pathology
Myocardial Reperfusion Injury / metabolism*
Myocardium / chemistry,  enzymology*,  metabolism
Physical Exertion / physiology
Protective Agents / metabolism,  pharmacology*
Random Allocation
Rats, Wistar
Ribonucleotides / metabolism,  pharmacology*
Ventricular Pressure / drug effects
Reg. No./Substance:
0/Blood Glucose; 0/Glucose Transporter Type 4; 0/Protective Agents; 0/Ribonucleotides; 3031-94-5/AICA ribonucleotide; 360-97-4/Aminoimidazole Carboxamide; 50-99-7/Glucose; 9005-79-2/Glycogen; EC Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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