Document Detail


4-Pentafluoroethylumbelliferyl-beta-D-glucoside as a new fluorogenic substrate for acid beta-D-glucosidase.
MedLine Citation:
PMID:  8740577     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
4-Pentafluoroethylumbelliferyl-beta-D-glucoside is proposed as an efficient substrate for human leukocyte acid beta-glucosidase. Its synthesis is described. This substrate was compared directly with 4-trifluoromethylumbelliferyl-beta-D-glucoside synthesized by us earlier and with 4-methylumbelliferyl-beta-D-glucoside which is commonly used for acid beta-glucosidase activity assay. The specific activity of acid beta-glucosidase with 4-pentafluoroethylumbelliferyl-beta-D-glucoside was 3- and 8-fold higher than it was with the substrates mentioned above. The kinetic parameters KM and VMAX for human leukocyte acid beta-glucosidase with the three substrates was determined. One possible application of the newly synthesized substrate is its use in the diagnosis of acid beta-glucosidase hereditary deficiency (Gaucher's disease).
Authors:
I V Tsvetkova; E A Karpova; T V Dudukina; Y V Voznyi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  248     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1997-01-15     Completed Date:  1997-01-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  125-33     Citation Subset:  IM    
Affiliation:
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
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MeSH Terms
Descriptor/Qualifier:
Fluorescent Dyes*
Gaucher Disease / enzymology
Glucosides / chemical synthesis,  metabolism*
Humans
Hydrogen-Ion Concentration
Hymecromone / chemistry
Kinetics
Leukocytes / enzymology
Magnetic Resonance Spectroscopy
Molecular Structure
Spectrophotometry
Umbelliferones / chemical synthesis,  metabolism*
beta-Glucosidase / metabolism*
Chemical
Reg. No./Substance:
0/4-pentafluoroethylumbelliferylglucoside; 0/Fluorescent Dyes; 0/Glucosides; 0/Umbelliferones; 90-33-5/Hymecromone; EC 3.2.1.21/beta-Glucosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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