| 4-Pentafluoroethylumbelliferyl-beta-D-glucoside as a new fluorogenic substrate for acid beta-D-glucosidase. | |
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MedLine Citation:
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PMID: 8740577 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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4-Pentafluoroethylumbelliferyl-beta-D-glucoside is proposed as an efficient substrate for human leukocyte acid beta-glucosidase. Its synthesis is described. This substrate was compared directly with 4-trifluoromethylumbelliferyl-beta-D-glucoside synthesized by us earlier and with 4-methylumbelliferyl-beta-D-glucoside which is commonly used for acid beta-glucosidase activity assay. The specific activity of acid beta-glucosidase with 4-pentafluoroethylumbelliferyl-beta-D-glucoside was 3- and 8-fold higher than it was with the substrates mentioned above. The kinetic parameters KM and VMAX for human leukocyte acid beta-glucosidase with the three substrates was determined. One possible application of the newly synthesized substrate is its use in the diagnosis of acid beta-glucosidase hereditary deficiency (Gaucher's disease). |
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Authors:
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I V Tsvetkova; E A Karpova; T V Dudukina; Y V Voznyi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 248 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1997-01-15 Completed Date: 1997-01-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 125-33 Citation Subset: IM |
Affiliation:
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Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescent Dyes* Gaucher Disease / enzymology Glucosides / chemical synthesis, metabolism* Humans Hydrogen-Ion Concentration Hymecromone / chemistry Kinetics Leukocytes / enzymology Magnetic Resonance Spectroscopy Molecular Structure Spectrophotometry Umbelliferones / chemical synthesis, metabolism* beta-Glucosidase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/4-pentafluoroethylumbelliferylglucoside; 0/Fluorescent Dyes; 0/Glucosides; 0/Umbelliferones; 90-33-5/Hymecromone; EC 3.2.1.21/beta-Glucosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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