| 4-Nonylphenol triggers apoptosis and affects 17-β-Estradiol receptors in calvarial osteoblasts. | |
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MedLine Citation:
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PMID: 22056370 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The present research examines the effects of 4-nonylphenol (4-NP) on mouse primary calvarial osteoblasts (COBs). Incubation of the cells with 4-NP at 10(-5)M and 10(-6)M striking decreased osteoblasts viability and phosphatidylserine (PS) exposure, measured by Annexin V, was greatly enhanced. In addition, an up-regulation of Bax/Bcl2 ratio with a drop in ΔΨm and an increase of cleaved caspase 9 and 3 was found, suggesting that the alkylphenol induced osteoblast death via the mitochondrial-dependent apoptotic pathway. Interestingly, treatment with 4-NP was also able to increase cleaved caspase 8 in parallel with the truncated active Bid (t-Bid) suggesting that 4-NP-mediated apoptosis depends on cross talk between the extrinsic and intrinsic pathways. It is of relevance, that the apoptotic effects of 4-NP overcame 17-β-Estradiol (17-β E(2)) induced-survival on osteoblasts. Also, the alkylphenol interfered with 17-β E(2) regulated estrogen receptors expression. |
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Authors:
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Maria Giovanna Sabbieti; Dimitrios Agas; Francesco Palermo; Gilberto Mosconi; Giorgio Santoni; Consuelo Amantini; Valerio Farfariello; Luigi Marchetti |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-25 |
Journal Detail:
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Title: Toxicology Volume: - ISSN: 1879-3185 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-11-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0361055 Medline TA: Toxicology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ireland Ltd. |
Affiliation:
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School of Biosciences and Biotechnology, University of Camerino, Camerino, MC, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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