| 4-Hydroxytamoxifen, an active metabolite of tamoxifen, does not alter the radiation sensitivity of MCF-7 breast carcinoma cells irradiated in vitro. | |
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MedLine Citation:
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PMID: 7949215 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of 4-hydroxytamoxifen (4OH-TAM), the potent anti-estrogenic metabolite of tamoxifen, on the radiosensitivity of MCF-7 cells irradiated in vitro was determined. Radiation dose response curves were generated for MCF-7 cells maintained and irradiated in phenol red-free medium containing 10(-10) M estradiol (E2) with or without 10(-7) M 4OH-TAM. Immediately after irradiation cells were transferred to medium containing 10(-10) ME2 supplemented with bovine serum to stimulate colony formation. Estradiol-stimulated cell proliferation was inhibited by 10(-7) M 4OH-TAM, but radiation sensitivity was not significantly altered (p > 0.3). Continued incubation in the absence of E2 for an additional 24 hours after irradiation likewise failed to alter the radiosensitivity of 4OH-TAM-treated MCF-7 cells. These studies indicate that growth-inhibitory concentrations of the anti-estrogen 4OH-TAM do not modify the in vitro radiation sensitivity of this line of human breast carcinoma cells. |
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Authors:
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J N Sarkaria; E M Miller; C J Parker; V C Jordan; R T Mulcahy |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Breast cancer research and treatment Volume: 30 ISSN: 0167-6806 ISO Abbreviation: Breast Cancer Res. Treat. Publication Date: 1994 |
Date Detail:
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Created Date: 1994-12-22 Completed Date: 1994-12-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8111104 Medline TA: Breast Cancer Res Treat Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 159-65 Citation Subset: IM |
Affiliation:
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Department of Human Oncology, University of Wisconsin, Madison 53792. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Breast Neoplasms
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pathology* Carcinoma / pathology* Cell Division / drug effects Female Humans Radiation Dosage Radiation Tolerance / drug effects* Tamoxifen / analogs & derivatives*, pharmacology Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA09471/CA/NCI NIH HHS; CA09614/CA/NCI NIH HHS; CA52686/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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10540-29-1/Tamoxifen; 68392-35-8/4-hydroxytamoxifen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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