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4-Hydroxynonenal Induces Mitochondrial-Mediated Apoptosis and Oxidative Stress in SH-SY5Y Human Neuronal Cells.
MedLine Citation:
PMID:  22118713     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Excessive and sustained increases in oxidative stress and apoptosis have been implicated in the pathogenesis of many diseases. In the present study, we demonstrated that 4-hydroxynonenal (4-HNE), a product of lipid peroxidation in a range of concentration (0.1-50 μM) showed cytotoxic effects on SH-SY5Y cell culture at a concentration > 5μM at 4 hr of exposure. 4-HNE dose-dependently decreased cell viability and significantly promoted reactive oxygen species formation and enhanced oxidative stress as reflected in the increased level of lipid peroxidation and catalase activity and decreased glutathione peroxidase activity as well as glutathione levels. 4-HNE-induced oxidative stress was associated with increased transcriptional and translational expressions of Bax and p53 in SH-SY5Y cells. Mitochondrial mediated apoptosis was confirmed by increased expression and activity of casapse-3. Our data demonstrate that 4-HNE induces neuronal cell death through abnormal expression of apoptotic markers (p53, Bax and caspase-3). Oxidative stress may be involved in the initial priming of SH-SY5Y cells to 4-HNE-induced cytotoxicity in vitro.
Authors:
Sunny O Abarikwu; Aditya B Pant; Ebenezer O Farombi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-24
Journal Detail:
Title:  Basic & clinical pharmacology & toxicology     Volume:  -     ISSN:  1742-7843     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101208422     Medline TA:  Basic Clin Pharmacol Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Basic & Clinical Pharmacology & Toxicology © 2011 Nordic Pharmacological Society.
Affiliation:
Department of Chemical Sciences, College of Natural Sciences, Redeemer's University, Redemption City, Ogun State, Nigeria. In Vitro Toxicology Laboratories, Indian Institute of Toxicology Research Lucknow, (Council of Scientific & Industrial Research) India. Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
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