Document Detail


4-aminobutyrate aminotransferase (ABAT): genetic and pharmacological evidence for an involvement in gastro esophageal reflux disease.
MedLine Citation:
PMID:  21552517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gastro-esophageal reflux disease (GERD) is partly caused by genetic factors. The underlying susceptibility genes are currently unknown, with the exception of COL3A1. We used three independent GERD patient cohorts to identify GERD susceptibility genes. Thirty-six families, demonstrating dominant transmission of GERD were subjected to whole genome microsatellite genotyping and linkage analysis. Five linked regions were identified. Two families shared a linked region (LOD 3.9 and 2.0) on chromosome 16. We used two additional independent GERD patient cohorts, one consisting of 219 trios (affected child with parents) and the other an adult GERD case control cohort consisting of 256 cases and 485 controls, to validate individual genes in the linked region through association analysis. Sixty six single nucleotide polymorphism (SNP) markers distributed over the nine genes present in the linked region were genotyped in the independent GERD trio cohort. Transmission disequilibrium test analysis followed by multiple testing adjustments revealed a significant genetic association for one SNP located in an intron of the gene 4-aminobutyrate aminotransferase (ABAT) (P(adj) = 0.027). This association did not replicate in the adult case-control cohort, possibly due to the differences in ethnicity between the cohorts. Finally, using the selective ABAT inhibitor vigabatrin (γ-vinyl GABA) in a dog study, we were able to show a reduction of transient lower esophageal sphincter relaxations (TLESRs) by 57.3 ± 11.4 % (p = 0.007) and the reflux events from 3.1 ± 0.4 to 0.8 ± 0.4 (p = 0.007). Our results demonstrate the direct involvement of ABAT in pathways affecting lower esophageal sphincter (LES) control and identifies ABAT as a genetic risk factor for GERD.
Authors:
Johan Jirholt; Bengt Asling; Paul Hammond; Geoffrey Davidson; Mikael Knutsson; Anna Walentinsson; Jörgen M Jensen; Anders Lehmann; Lars Agreus; Maria Lagerström-Fermer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-28
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-05-09     Completed Date:  2011-08-23     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e19095     Citation Subset:  IM    
Affiliation:
AstraZeneca R&D, Mölndal, Sweden.
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MeSH Terms
Descriptor/Qualifier:
4-Aminobutyrate Transaminase / genetics*
Adolescent
Adult
Animals
Case-Control Studies
Dogs
Esophageal Sphincter, Lower / metabolism,  physiopathology
Female
Gastroesophageal Reflux / drug therapy*,  genetics*,  physiopathology
Genetic Linkage
Genetic Predisposition to Disease
Humans
Male
Reproducibility of Results
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
EC 2.6.1.19/4-Aminobutyrate Transaminase
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