| 3beta-taraxerol of Mangifera indica, a PI3K dependent dual activator of glucose transport and glycogen synthesis in 3T3-L1 adipocytes. | |
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MedLine Citation:
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PMID: 20026188 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The present study focuses on identifying and developing an anti-diabetic molecule from plant sources that would effectively combat insulin resistance through proper channeling of glucose metabolism involving glucose transport and storage. METHODS: Insulin-stimulated glucose uptake formed the basis for isolation of a bioactive molecule through column chromatography followed by its characterization using NMR and mass spectroscopic analysis. Mechanism of glucose transport and storage was evaluated based on the expression profiling of signaling molecules involved in the process. RESULTS: The study reports (i) the isolation of a bioactive compound 3beta-taraxerol from the ethyl acetate extract (EAE) of the leaves of Mangifera indica (ii) the bioactive compound exhibited insulin-stimulated glucose uptake through translocation and activation of the glucose transporter (GLUT4) in an IRTK and PI3K dependent fashion. (iii) the fate of glucose following insulin-stimulated glucose uptake was ascertained through glycogen synthesis assay that involved the activation of PKB and suppression of GSK3beta. GENERAL SIGNIFICANCE: This study demonstrates the dual activity of 3beta-taraxerol and the ethyl acetate extract of Mangifera indica as a glucose transport activator and stimulator of glycogen synthesis. 3beta-taraxerol can be validated as a potent candidate for managing the hyperglycemic state. |
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Authors:
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Kadapakkam Nandabalan Sangeetha; Sundaresan Sujatha; Velusamy Shanmuganathan Muthusamy; Singaravel Anand; Nirmal Nithya; Devadasan Velmurugan; Arun Balakrishnan; Baddireddi Subhadra Lakshmi |
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Publication Detail:
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Type: Journal Article Date: 2009-12-21 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1800 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-22 Completed Date: 2010-05-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 359-66 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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Centre for Biotechnology, Anna University, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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drug effects,
metabolism* 3T3 Cells / drug effects, metabolism* Adipocytes / cytology, drug effects, enzymology* Animals Blood Glucose / metabolism Cell Survival / drug effects Deoxyglucose / metabolism* Diabetes Mellitus, Type 2 / epidemiology, physiopathology Enzyme Activation Glucose / metabolism* Glycogen / biosynthesis* Humans Insulin Resistance Mice Oleanolic Acid / analogs & derivatives*, isolation & purification, pharmacology Plant Extracts / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Mangifera indica extract; 0/Plant Extracts; 127-22-0/taraxerol; 154-17-6/Deoxyglucose; 50-99-7/Glucose; 508-02-1/Oleanolic Acid; 9005-79-2/Glycogen; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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