Document Detail


BALB/c 3T3 cell transformation assay for the prediction of carcinogenic potential of chemicals and environmental mixtures.
MedLine Citation:
PMID:  20226850     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The prediction of the carcinogenic risk for humans is mostly based on animal experiments. For the last 20 years, however, the scientific community has paid great attention to alternative strategies in compliance with common moral and ethical values. The new European chemical regulation REACH (Reg. EC 1907/2006) requires the performance of new studies in vertebrates only as a last resort. REACH asks for the development of validated in vitro protocols that can replace, in the medium to the long term, animal bioassays. An in vitro cell transformation assay (CTA) is proposed as an alternative to in vivo carcinogenicity testing. This assay is reported in the list of accepted methods for REACH (Reg. EC 440/2008). The BALB/c 3T3 model represents one of the most well-known CTAs and is regarded as a useful tool to screen single chemicals or complex mixtures for carcinogenicity prediction. In this study we used a modified protocol to highlight the transforming potential of three single compounds, ethinylestradiol (EE), azathioprine (AZA-T), melphalan, and two polychlorinated biphenyls (PCBs) mixtures, which are known or suspected to be human carcinogens. We also evaluated the activity of the antioxidant alpha-lipoic acid (ALA), a promising tumor chemopreventive. A significant increase in transformation frequency was observed when the BALB/c 3T3 cells were exposed to EE, AZA-T or melphalan as well as after PCBs treatment. On the contrary, ALA did not induce any increase of foci occurrence. Our results confirm the suitability of the improved protocol to discriminate carcinogenic compounds and support the use of BALB/c 3T3 cell transformation assay as a possible alternative to predict carcinogenic risk to humans.
Authors:
Maria Grazia Mascolo; Stefania Perdichizzi; Francesca Rotondo; Elena Morandi; Angela Guerrini; Paola Silingardi; Monica Vaccari; Sandro Grilli; Annamaria Colacci
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-11
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  24     ISSN:  1879-3177     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-17     Completed Date:  2010-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  1292-300     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Environmental Carcinogenesis and Risk Assessment, Environmental Protection and Health Prevention Agency - Emilia-Romagna Region (ER-EPA), Viale Filopanti 22, 40126 Bologna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Azathioprine / toxicity
BALB 3T3 Cells
Carcinogenicity Tests / methods*
Carcinogens / toxicity*
Cell Transformation, Neoplastic / chemically induced*
Environmental Pollutants / toxicity
Ethinyl Estradiol / toxicity
Melphalan / toxicity
Mice
Polychlorinated Biphenyls / toxicity
Chemical
Reg. No./Substance:
0/Carcinogens; 0/Environmental Pollutants; 0/Polychlorinated Biphenyls; 148-82-3/Melphalan; 446-86-6/Azathioprine; 57-63-6/Ethinyl Estradiol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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