Document Detail

[3H]Allicin: preparation and applications.
MedLine Citation:
PMID:  15265743     Owner:  NLM     Status:  MEDLINE    
Allicin (diallylthiosulfinate), the active substance of garlic, has been shown to possess a variety of biological activities. Mechanistic and pharmacokinetic studies of allicin and its derivatives raise the need for a labeled compound. However, labeling of this volatile and unstable liquid requires delicate handling. Here, we describe a simple method for the preparation of (3)H-labeled allicin. This was achieved by applying synthetic [(3)H]alliin ([2,3-(3)H]allylcysteine sulfoxide) to a column containing immobilized alliinase [EC] from garlic. Purification of [(3)H]allicin was done by differential adsorbtion of the reaction components on a neutral polystyrene resin, Porapak Q. Thiol-containing compounds are known to be the main target of allicin. In this work we demonstrated that [(3)H]allicin can be used for the synthesis of labeled [(3)H]allylmercapto derivatives of SH peptides and proteins. Thus, we prepared [(3)H]S-allylmercaptoglutathione which can be used in metabolic studies. Moreover, we showed that incubation of alliinase with [(3)H]allicin led to modification of 1.4 cysteine residues per subunit of the enzyme.
T Miron; T Bercovici; A Rabinkov; M Wilchek; D Mirelman
Related Documents :
15812873 - Diversity oriented synthesis of hispanane-like terpene derivatives from (r)-(+)-sclareo...
12213493 - Oxylipin profiling in pathogen-infected potato leaves.
271453 - Discoloration of dental pellicle by tannic acid.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  331     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-07-21     Completed Date:  2005-02-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  364-9     Citation Subset:  IM    
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromatography, Gel
Sulfinic Acids / chemical synthesis*,  isolation & purification
Reg. No./Substance:
0/Sulfinic Acids; 10028-17-8/Tritium; 539-86-6/allicin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Identification of disulfide bonds and site-specific glycosylation in chicken alpha1-acid glycoprotei...
Next Document:  Colorimetric ferrozine-based assay for the quantitation of iron in cultured cells.