Document Detail

3D-topography of cell nuclei in a vertebrate retina--a confocal and two-photon microscopic study.
MedLine Citation:
PMID:  20122964     Owner:  NLM     Status:  MEDLINE    
We demonstrate methods to simultaneously acquire and evaluate the pattern of cell nuclei in the three cell layers of the vertebrate retina as an aspect of its functional morphology. 3D-position, shape and quantity of fluorescence-labelled cell nuclei are measured using laser scanning microscopy at several retinal locations, the pros and cons of single and two-photon excitation are compared. Subsequently topographies of all discriminable morphotypes are calculated via linear interpolation of local countings. In addition derived maps are calculated correlating density- and layer thickness-distributions to demonstrate the potential of 3D-morphometry in the retina. In the European anchovy Engraulis encrasicolus L. (Engraulididae, Teleostei) the angular density of all involved cell types varies considerably with the location in the hemispherical coordinate system. All cells belonging to the photopic system show a density peak in the ventro-temporal quadrant, suggesting acute vision in the frontal binocular visual field. A second, less pronounced maximum is found nasally.
Petra Christiane Koch; Christian Seebacher; Martin Hess
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-01
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  188     ISSN:  1872-678X     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-29     Completed Date:  2010-06-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  127-40     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Biozentrum LMU M?nchen, Biology 1, M?nchen, Germany.
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MeSH Terms
Cell Nucleus / ultrastructure*
Fishes / anatomy & histology
Microscopy, Confocal
Microscopy, Fluorescence, Multiphoton
Retina / ultrastructure*
Retinal Ganglion Cells / ultrastructure*

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