| 3D tomography reveals connections between the phagophore and endoplasmic reticulum. | |
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MedLine Citation:
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PMID: 19855179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Autophagosomes have been reported to form in the vicinity of the endoplasmic reticulum (ER). In many cases, the phagophore membrane is observed between two cisternae of rough ER, but it is not known whether these two membranes are directly connected. To investigate the relationship of the phagophore membrane and the ER, we used electron microscopic tomography of serum and amino acid starved normal rat kidney cells. The cells were fixed in glutaraldehyde and reduced osmium tetroxide and embedded in Epon. Dual axis tilt image series were acquired from two successive 250-nm sections. To analyze the three-dimensional (3D) morphology of phagophores and the associated rough ER, 3D tomograms were used to model the ER and phagophore membranes. The tomographic reconstructions revealed connections between the phagophore/autophagosome membrane and the closely located ER cisternae, especially with the ER located inside the autophagosome. The connections were typically formed by narrow extensions from the phagophore/autophagosome to the ER. This finding has potential implications on the origin of autophagosome membranes, and on the mechanism of phagophore membrane extension. In addition, we observed lipid droplets in very close contact with the phagophores/autophagosomes. |
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Authors:
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P?ivi Yl?-Anttila; Helena Vihinen; Eija Jokitalo; Eeva-Liisa Eskelinen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-08 |
Journal Detail:
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Title: Autophagy Volume: 5 ISSN: 1554-8635 ISO Abbreviation: Autophagy Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-12-03 Completed Date: 2010-02-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101265188 Medline TA: Autophagy Country: United States |
Other Details:
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Languages: eng Pagination: 1180-5 Citation Subset: IM |
Affiliation:
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University of Helsinki, Department of Biological and Environmental Sciences, Division of Biochemistry, Helsinki, Finland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Endoplasmic Reticulum / metabolism*, ultrastructure Intracellular Membranes / metabolism, ultrastructure Lipid Metabolism Mice Models, Biological Phagosomes / metabolism*, ultrastructure Rats Tomography / methods* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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