Document Detail


3D structural and functional characterization of the transition from Hodgkin to Reed-Sternberg cells.
MedLine Citation:
PMID:  20810259     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent research using an innovative 3D quantitative FISH approach of nuclear remodelling associated with the transition from mononuclear Hodgkin to diagnostic multinuclear Reed-Sternberg cells revealed profound changes in the 3D nuclear organization of telomeres. Analogous 3D telomere dynamics were identified in Hodgkin's lymphoma derived cell-lines and diagnostic patient biopsies. These changes were observed in both, EBV positive and EBV-negative Hodgkin's lymphoma and independent of the age of the patients at presentation. Compared to mononuclear Hodgkin cells, multinuclear Reed-Sternberg cells are characterized by a highly significant increase of telomere aggregates, often composed of very short telomeres, telomere shortening and loss. RS-cells with telomere free "ghost" nuclei are regularly observed. The telomere protecting shelterin complex appears to be disrupted and deregulation of DNA-repair mechanisms is observed. Our findings are consistent with the hypothesis that distinct 3D telomere changes and shelterin disruption represent a common pathogenetic denominator in the generation of Reed-Sternberg cells.
Authors:
Hans Knecht; Silke Brüderlein; Sabine Mai; Peter Möller; Bassem Sawan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-08-06
Journal Detail:
Title:  Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft     Volume:  192     ISSN:  1618-0402     ISO Abbreviation:  Ann. Anat.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-17     Completed Date:  2010-10-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100963897     Medline TA:  Ann Anat     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  302-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier GmbH. All rights reserved.
Affiliation:
Division d'Hématologie, CHUS, Université de Sherbrooke, Sherbrooke, Québec, Canada. hans.knecht@usherbrooke.ca
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / genetics,  ultrastructure
Hodgkin Disease / genetics,  pathology*
Humans
Imaging, Three-Dimensional
Reed-Sternberg Cells / pathology,  ultrastructure*
Telomere / ultrastructure*
Grant Support
ID/Acronym/Agency:
//Canadian Institutes of Health Research

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