Document Detail


A 3D map of the yeast kinetochore reveals the presence of core and accessory centromere-specific histone.
MedLine Citation:
PMID:  24076245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The budding yeast kinetochore is ~68 nm in length with a diameter slightly larger than a 25 nm microtubule. The kinetochores from the 16 chromosomes are organized in a stereotypic cluster encircling central spindle microtubules. Quantitative analysis of the inner kinetochore cluster (Cse4, COMA) reveals structural features not apparent in singly attached kinetochores. The cluster of Cse4-containing kinetochores is physically larger perpendicular to the spindle axis relative to the cluster of Ndc80 molecules. If there was a single Cse4 (molecule or nucleosome) at the kinetochore attached to each microtubule plus end, the cluster of Cse4 would appear geometrically identical to Ndc80. Thus, the structure of the inner kinetochore at the surface of the chromosomes remains unsolved. We have used point fluorescence microscopy and statistical probability maps to deduce the two-dimensional mean position of representative components of the yeast kinetochore relative to the mitotic spindle in metaphase. Comparison of the experimental images to three-dimensional architectures from convolution of mathematical models reveals a pool of Cse4 radially displaced from Cse4 at the kinetochore and kinetochore microtubule plus ends. The pool of displaced Cse4 can be experimentally depleted in mRNA processing pat1Δ or xrn1Δ mutants. The peripheral Cse4 molecules do not template outer kinetochore components. This study suggests an inner kinetochore plate at the centromere-microtubule interface in budding yeast and yields information on the number of Ndc80 molecules at the microtubule attachment site.
Authors:
Julian Haase; Prashant K Mishra; Andrew Stephens; Rachel Haggerty; Cory Quammen; Russell M Taylor; Elaine Yeh; Munira A Basrai; Kerry Bloom
Related Documents :
25109385 - The condensin component ncapg2 regulates microtubule-kinetochore attachment through rec...
24171835 - Complex small supernumerary marker chromosomes - an update.
24009515 - Comparative anatomy of chromosomal domains with imprinted and non-imprinted allele-spec...
24270525 - Effects of marked chromosome sections on quantitative traits in the mouse.
16501575 - High mutation rates have driven extensive structural polymorphism among human y chromos...
15307845 - Short tandem repeat analysis to monitor chimerism in macaca fascicularis.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural     Date:  2013-09-26
Journal Detail:
Title:  Current biology : CB     Volume:  23     ISSN:  1879-0445     ISO Abbreviation:  Curr. Biol.     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-11     Completed Date:  2014-05-16     Revised Date:  2014-10-09    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1939-44     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Ltd. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Centromere / metabolism*
Chromosomal Proteins, Non-Histone / metabolism*
DNA-Binding Proteins / metabolism*
Exoribonucleases / genetics
Histones / metabolism*
Kinetochores / metabolism*
Microtubule-Associated Proteins / genetics
Microtubules
Nuclear Proteins / metabolism*
Protein Structure, Quaternary
RNA-Binding Proteins / genetics
Saccharomyces cerevisiae / genetics,  metabolism*
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Spindle Apparatus / metabolism
Grant Support
ID/Acronym/Agency:
P41 EB002025/EB/NIBIB NIH HHS; R37 GM032238/GM/NIGMS NIH HHS; R37 GM32238/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/CSE4 protein, S cerevisiae; 0/Chromosomal Proteins, Non-Histone; 0/DNA-Binding Proteins; 0/Histones; 0/Microtubule-Associated Proteins; 0/Nuclear Proteins; 0/PAT1 protein, S cerevisiae; 0/RNA-Binding Proteins; 0/Saccharomyces cerevisiae Proteins; 0/TID3 protein, S cerevisiae; EC 3.1.-/Exoribonucleases; EC 3.1.11.-/KEM1 protein, S cerevisiae
Comments/Corrections
Comment In:
Curr Biol. 2013 Oct 7;23(19):R875-8   [PMID:  24112982 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Dissociation of Circadian and Circatidal Timekeeping in the Marine Crustacean Eurydice pulchra.
Next Document:  Cognitive Unbinding: A Neuroscientific Paradigm of General Anesthesia and Related States of Unconsci...