| 3,3'-diindolylmethane inhibits prostate cancer development in the transgenic adenocarcinoma mouse prostate model. | |
| | |
MedLine Citation:
|
PMID: 21061271 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
3,3'-Diindolylmethane (DIM) is a major in vivo derivative of indole-3-carbinol, which is present in cruciferous vegetables and has been reported to possess anti-carcinogenic properties. In the present study, we examined whether DIM inhibits the development of prostate cancer using the transgenic adenocarcinoma mouse prostate (TRAMP) model. DIM feeding inhibited prostate carcinogenesis in TRAMP mice, reduced the number of cells expressing the SV40 large tumor antigen and proliferating cell nuclear antigen, and increased the number of terminal dUTP nick-end labeling-positive cells in the dorsolateral lobes of the prostate. Additionally, DIM feeding reduced the expression of cyclin A, cyclin-dependent kinase (CDK)2, CDK4, and Bcl-xL, and increased p27 and Bax expression. To assess the mechanisms by which DIM induces apoptosis, LNCaP and DU145 human prostate cancer cells were cultured with various concentrations of DIM. DIM induced a substantial reduction in the numbers of viable cells and induced apoptosis in LNCaP and DU145 cells. DIM increased the cleavage of caspase-9, -7, -3, and poly (ADP-ribose) polymerase (PARP). DIM increased mitochondrial membrane permeability and the translocation of cytochrome c and Smac/Diablo from the mitochondria. Additionally, DIM induced increases in the levels of cleaved caspase-8, truncated Bid, Fas, and Fas ligand, and the caspase-8 inhibitor Z-IETD-FMK was shown to mitigate DIM-induced apoptosis and the cleavage of caspase-3, PARP, and Bid. These results indicate that DIM inhibits prostate carcinogenesis via induction of apoptosis and inhibition of cell cycle progression. DIM induces apoptosis in prostate cancer cells via the mitochondria- and death receptor-mediated pathways. © 2010 Wiley-Liss, Inc. |
| | |
Authors:
|
Han Jin Cho; So Young Park; Eun Ji Kim; Jin-Kyung Kim; Jung Han Yoon Park |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2010-11-8 |
Journal Detail:
|
Title: Molecular carcinogenesis Volume: - ISSN: 1098-2744 ISO Abbreviation: - Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8811105 Medline TA: Mol Carcinog Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Food Science and Nutrition, Hallym University, Chuncheon, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ingested RNA interference for managing the populations of the Colorado potato beetle, Leptinotarsa d...
Next Document: Incidence of work injuries amongst Danish adolescents and their association with work environment fa...