Document Detail


A 33 bp minisatellite repeat upstream of the 'mutated in colon cancer' gene at chromosome 5q21.
MedLine Citation:
PMID:  9694282     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have found a 33 bp minisatellite repeat in the 5'-flanking region of the mutated in colon cancer (MCC) gene at chromosome 5q21. Southern blot experiments demonstrated the locus specificity of the repeat. The number of repeat units varied between 5 and 11 with a heterozygosity of 0.56. The sequence 5'-AGG AGT GTG AAT GGG GCA TAG TGA ATG AGG GGA-3' of the repeat units does not match the consensus sequence of chi-related minisatellites. The minisatellite is not expressed as part of a gene transcription unit. However, it can be used as a tool for the detection of allelic changes at chromosome 5q21 on standard agarose gels.
Authors:
P Bugert; C Kenck; G Kovacs
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  19     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-11-13     Completed Date:  1998-11-13     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  1362-5     Citation Subset:  IM    
Affiliation:
Dept. of Urology, Ruprecht-Karls-University of Heidelberg, Germany. peter.bugert@urz.uni-heidelberg.de
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 5*
Colonic Neoplasms / genetics*
Gene Expression
Humans
Microsatellite Repeats*
Molecular Sequence Data
Polymorphism, Genetic
Proteins / genetics*
Tumor Suppressor Proteins*
Chemical
Reg. No./Substance:
0/Proteins; 0/Tumor Suppressor Proteins; 137764-10-4/MCC protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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