| 3,3'-Diindolylmethane negatively regulates Cdc25A and induces a G2/M arrest by modulation of microRNA 21 in human breast cancer cells. | |
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MedLine Citation:
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PMID: 20724916 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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3,3'-Diindolylmethane (DIM) is a potential chemopreventive phytochemical derived from Brassica vegetables. In this study, we assessed the effects of DIM on cell cycle regulation in both estrogen-dependent MCF-7 and estrogen receptor negative p53 mutant MDA-MB-468 human breast cancer cells. In-vitro culture studies showed that DIM dose dependently inhibited the proliferation of both cells. In addition, in-vivo xenograft model showed that DIM strongly inhibited the development of human breast tumors. Fluorescence activated cell sorter analysis showed a DIM-mediated G2/M cell cycle arrest in MCF-7 and MDA-MB-468 cells. Western blot analysis showed that DIM downregulated the expression of cyclin-dependent kinases 2 and 4 and Cdc25A, which plays an important role in G2/M phase. Furthermore, treatment of MCF-7 cells with DIM, which increased microRNA 21 expression, caused a downregulation of Cdc25A, resulting in an inhibition of breast cancer cell proliferation. Taken together, our data show that DIM is able to stop the cell cycle progression of human breast cancer cells regardless of their estrogen-dependence and p53 status, by differentially modulating cell cycle regulatory pathways. The modulation of microRNA 21 mediates the DIM cell cycle regulator effect in MCF-7 cells. |
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Authors:
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Yucui Jin; Xianghong Zou; Xiaoling Feng |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Retracted Publication |
Journal Detail:
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Title: Anti-cancer drugs Volume: 21 ISSN: 1473-5741 ISO Abbreviation: Anticancer Drugs Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-02 Completed Date: 2010-12-22 Revised Date: 2011-03-07 |
Medline Journal Info:
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Nlm Unique ID: 9100823 Medline TA: Anticancer Drugs Country: England |
Other Details:
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Languages: eng Pagination: 814-22 Citation Subset: IM |
Affiliation:
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Capital Normal University, Beijing, China. lantianmeiyu1985@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticarcinogenic Agents / administration & dosage, pharmacology Antineoplastic Agents / administration & dosage, pharmacology* Blotting, Western Breast Neoplasms / drug therapy*, pathology Cell Division / drug effects Cell Line, Tumor Cell Proliferation / drug effects Dose-Response Relationship, Drug Female G2 Phase / drug effects Gene Expression Regulation, Neoplastic / drug effects Humans Indoles / administration & dosage, pharmacology* Mice Mice, Inbred BALB C Mice, Nude MicroRNAs / metabolism* Xenograft Model Antitumor Assays cdc25 Phosphatases / drug effects, genetics |
| Grant Support | |
ID/Acronym/Agency:
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CA113579/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anticarcinogenic Agents; 0/Antineoplastic Agents; 0/Indoles; 0/MicroRNAs; 0/mirn21 microRNA, rat; 1968-05-4/3,3'-diindolylmethane; EC 3.1.3.48/CDC25A protein, human; EC 3.1.3.48/cdc25 Phosphatases |
| Comments/Corrections | |
Retraction In:
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Anticancer Drugs. 2011 Mar;22(3):303
[PMID:
21282984
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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