| The 31P NMR visibility of ATP in perfused rat liver remains about 90%, unaffected by changes of metabolic state. | |
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MedLine Citation:
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PMID: 1510935 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The 31P NMR visibility of ATP of the perfused rat liver was tested over a wide range of metabolic conditions, including normoxic and hypoxic perfusions, fructose loads, and various intervals of normothermic ischemia, for both ad libitum fed and 24-h fasted rats. The 31P NMR signal of ATP was compared to the concentration of ATP determined by enzymatic assays on liver biopsies performed at the end of NMR acquisition. In a first series of experiments, the NMR resonance of intracellular ATP was quantitated in absolute terms by applying the 1H NMR water signal as internal reference: during normoxic and hypoxic perfusions, a constant amount of ATP (0.43 +/- 0.19 mM, mean +/- SD), approximately 12% of the cellular ATP, is not detected by NMR. Nevertheless, there is a high correlation (slope = 0.96 +/- 0.09; r2 = 0.93) between the measurements of ATP by 31P NMR spectroscopy and by biochemical analysis. In a second series of experiments, there was a highly significant correlation between the NMR and analytical biochemical measurements of ATP for whole range of metabolic states, i.e., fructose loads (1.0-10 mM) and various intervals of normothermic ischemia (ranging from 2 to 12 min), indicating unchanged ATP visibility. Thus, as opposed to the studies of Murphy et al. [Murphy, E., et al. (1988) Biochemistry 27, 526-528], it is concluded that ATP at 37 degrees C remains almost entirely visible in the perfused rat liver, also during ischemia. |
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Authors:
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S Masson; B Quistorff |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry Volume: 31 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1992-09-29 Completed Date: 1992-09-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7488-93 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Panum Institute, Copenhagen, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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metabolism Adenosine Monophosphate / metabolism Adenosine Triphosphate / analysis, metabolism* Animals Anoxia / metabolism Ischemia / metabolism Liver / blood supply, metabolism* Magnetic Resonance Spectroscopy / methods Male Perfusion / instrumentation, methods Phosphates / metabolism Phosphorus Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Phosphates; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 61-19-8/Adenosine Monophosphate; 7723-14-0/Phosphorus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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