Document Detail


31P NMR spectroscopy of rat organs, in situ, using chronically implanted radiofrequency coils.
MedLine Citation:
PMID:  6584867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A technique for making 31P NMR spectroscopic measurements in rat kidney, heart, and liver in vivo is presented. Two-turn solenoid coils were surgically implanted around the organ sufficiently in advance of NMR experiments to allow recovery of the animal. These chronically implanted coils allowed acquisition of high-resolution spectra at 40.5 and 97.3 MHz. No resolution improvement occurred at the higher field. Spectra were stable for up to 24 hr, during which time a variety of experiments could be performed. By accumulating spectra at 10-min intervals, the effects of intraperitoneal fructose injections were monitored; in kidney and liver, a rapid increase in sugar phosphates at the expense of Pi and ATP resulted. Fructose had no effect on heart metabolite levels. Spectra from the heart in vivo were obtained at systole and diastole by gating the spectrometer to the aortic pressure wave; no differences in phosphate metabolites were detected. Finally, saturation transfer techniques were used to monitor the rate of ATP synthesis in the kidney. The unidirectional rate constant for the conversion of Pi to ATP was 0.12 +/- 0.03 sec-1.
Authors:
A P Koretsky; S Wang; J Murphy-Boesch; M P Klein; T L James; M W Weiner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  80     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-05-11     Completed Date:  1984-05-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7491-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / metabolism
Adenosine Triphosphate / metabolism
Animals
Fructose / pharmacology
Heart / physiology
Kidney / drug effects,  metabolism*
Liver / drug effects,  metabolism*
Magnetic Resonance Spectroscopy / instrumentation,  methods
Myocardium / metabolism*
Phosphocreatine / metabolism
Radio Waves
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
30237-26-4/Fructose; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 67-07-2/Phosphocreatine
Comments/Corrections

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