| 31P NMR spectroscopic studies of the effects of cyclophosphamide on perfused RIF-1 tumor cells. | |
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MedLine Citation:
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PMID: 8057794 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine whether direct cellular effects of chemotherapy are responsible for 31P NMR spectral changes observed in treated tumors in vivo, RIF-1 fibrosarcoma cells were examined in vitro before, during, and after treatment with 4-hydroperoxycyclophosphamide (4-HC), an activated form of cyclophosphamide. When RIF-1 cells were treated with 4-HC in a metabolically stable but nonproliferating state, the 31P NMR spectra were identical with those of untreated cells for up to 70 h. When actively proliferating RIF-1 cells were treated with 4-HC, the intensities of the nucleotide triphosphate resonances, which increased linearly during control cell growth, remained constant for 50 h or longer. These studies demonstrate that the bioenergetic improvement observed following treatment of RIF-1 tumors in vivo [S.-J. Li, J.P. Wehrle, S.S. Rajan, R.G. Steen, J.D. Glickson, and J. Hilton, Cancer Res. 48, 4736 (1988)] does not result from direct effects of cyclophosphamide metabolites on RIF-1 cell metabolism, but rather from indirect effects of treatment on tumor or host physiology. |
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Authors:
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N R Aiken; K A McGovern; C E Ng; J P Wehrle; J D Glickson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 31 ISSN: 0740-3194 ISO Abbreviation: Magn Reson Med Publication Date: 1994 Mar |
Date Detail:
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Created Date: 1994-09-14 Completed Date: 1994-09-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 241-7 Citation Subset: IM |
Affiliation:
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Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division Cyclophosphamide / analogs & derivatives*, pharmacology Drug Screening Assays, Antitumor / methods* Fibrosarcoma / pathology* Magnetic Resonance Spectroscopy Mice Phosphorus Isotopes Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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5P30CAO697330/CA/NCI NIH HHS; R01-CA51950/CA/NCI NIH HHS; T32-CA09630/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphorus Isotopes; 39800-16-3/perfosfamide; 50-18-0/Cyclophosphamide |
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