| 31P NMR measurements of the ADP concentration in yeast cells genetically modified to express creatine kinase. | |
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MedLine Citation:
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PMID: 2185836 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rabbit muscle creatine kinase has been introduced into the yeast Saccharomyces cerevisiae by transforming cells with a multicopy plasmid containing the coding sequence for the enzyme under the control of the yeast phosphoglycerate kinase promoter. The transformed cells showed creatine kinase activities similar to those found in mammalian heart muscle. 31P NMR measurements of the near-equilibrium concentrations of phosphocreatine and cellular pH together with measurements of the total extractable concentrations of phosphocreatine and creatine allowed calculation of the free ADP/ATP ratio in the cell. The calculated ratio of approximately 2 was considerably higher than the ratio of between 0.06 and 0.1 measured directly in cell extracts. |
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Authors:
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K Brindle; P Braddock; S Fulton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry Volume: 29 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1990 Apr |
Date Detail:
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Created Date: 1990-06-12 Completed Date: 1990-06-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3295-302 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Oxford, England. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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metabolism* Animals Creatine Kinase / genetics*, metabolism Magnetic Resonance Spectroscopy / methods Plasmids Rabbits Rats Saccharomyces cerevisiae / genetics*, metabolism Transformation, Genetic* |
| Chemical | |
Reg. No./Substance:
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58-64-0/Adenosine Diphosphate; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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