Document Detail


31P NMR measurements of the ADP concentration in yeast cells genetically modified to express creatine kinase.
MedLine Citation:
PMID:  2185836     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rabbit muscle creatine kinase has been introduced into the yeast Saccharomyces cerevisiae by transforming cells with a multicopy plasmid containing the coding sequence for the enzyme under the control of the yeast phosphoglycerate kinase promoter. The transformed cells showed creatine kinase activities similar to those found in mammalian heart muscle. 31P NMR measurements of the near-equilibrium concentrations of phosphocreatine and cellular pH together with measurements of the total extractable concentrations of phosphocreatine and creatine allowed calculation of the free ADP/ATP ratio in the cell. The calculated ratio of approximately 2 was considerably higher than the ratio of between 0.06 and 0.1 measured directly in cell extracts.
Authors:
K Brindle; P Braddock; S Fulton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry     Volume:  29     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-06-12     Completed Date:  1990-06-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3295-302     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Oxford, England.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / metabolism*
Animals
Creatine Kinase / genetics*,  metabolism
Magnetic Resonance Spectroscopy / methods
Plasmids
Rabbits
Rats
Saccharomyces cerevisiae / genetics*,  metabolism
Transformation, Genetic*
Chemical
Reg. No./Substance:
58-64-0/Adenosine Diphosphate; EC 2.7.3.2/Creatine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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