Document Detail

31P-MRS-based determination of brain intracellular and interstitial pH: its application to in vivo H+ compartmentation and cellular regulation during hypoxic/ischemic conditions.
MedLine Citation:
PMID:  11059809     Owner:  NLM     Status:  MEDLINE    
In the last decade, significant progress has been made in the characterization of pH regulation in nervous tissue in vitro. However, little work has been directed at understanding how pH regulatory mechanisms function in vivo. We are interested in how ischemic acidosis can effect pH regulation and modulate the extent of post-ischemic brain damage. We used 31P-MRS to determine normal in vivo pH(i) and pH(e) simultaneously in both the isolated canine brain and the intact rat brain. We observed that the 31P(i) peak in the 31P-MRS spectrum is heterogeneous and can be deconvoluted into a number of discrete constituent peaks. In a series of experiments, we identified these peaks as arising from either extracellular or intracellular sources. In particular, we identified the peak representing the neurons and astrocytes and showed that they maintain different basal pH (6.95 and 7.05, respectively) and behave differently during hypoxic/ischemic episodes.
D B Kintner; M K Anderson; J H Fitzpatrick; K A Sailor; D D Gilboe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Neurochemical research     Volume:  25     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2001-02-14     Completed Date:  2001-02-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1385-96     Citation Subset:  IM    
University of Wisconsin Medical School, Department of Neurological Surgery, Madison 53716-1572, USA.
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MeSH Terms
Brain / metabolism*
Hydrogen-Ion Concentration*
Hypoxia-Ischemia, Brain / metabolism*
Magnetic Resonance Spectroscopy / methods
Grant Support
Reg. No./Substance:

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