| The 3' untranslated region of the human interferon-beta mRNA has an inhibitory effect on translation. | |
| | |
MedLine Citation:
|
PMID: 3476924 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In vitro-transcribed human interferon-beta (IFN-beta) mRNA, which contains all the sequence of the natural molecule, is poorly translated in a reticulocyte lysate or when injected in Xenopus oocytes. This low level of translation is due to an inhibition by the 5' and 3' untranslated regions (UTRs). Indeed, the replacement of these sequences by those of Xenopus beta-globin mRNA dramatically increases the translational efficiency of the mRNA, especially in oocytes. This phenomenon is not due to a difference in mRNA stability since both native and chimeric mRNAs remain undegraded, at least during the translation period considered. Construction of different chimeric molecules having various combinations of 5' and 3' UTRs from IFN-beta or Xenopus beta-globin mRNA or a small sequence of SP6 polylinker as 5' UTR has revealed that the 3' UTR of IFN-beta in itself has a pronounced inhibitory effect on translation in the two translation systems from animal cells. Indeed, the addition of this 3' UTR at the 3' end of the coding region of a chicken lysozyme mRNA also causes a large decrease of its translational capacity in both systems. However, the nature of the 5' noncoding sequence influences the degree of translation inhibition exerted by the 3' UTR. Remarkably, we observed no difference in translation level when the different mRNAs were tested in a wheat germ extract. |
| | |
Authors:
|
V Kruys; M Wathelet; P Poupart; R Contreras; W Fiers; J Content; G Huez |
Related Documents
:
|
20017904 - Upstream orf affects mycn translation depending on exon 1b alternative splicing. 14612424 - Mtor-dependent regulation of ribosomal gene transcription requires s6k1 and is mediated... 15734734 - Homocysteine down-regulates cellular glutathione peroxidase (gpx1) by decreasing transl... 18793384 - Yb-1 promotes microtubule assembly in vitro through interaction with tubulin and microt... 17493814 - Nfat signaling and the invention of vertebrates. 9700074 - Expression of psba genes produces prominent 5' psba mrna fragments in synechococcus sp.... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 84 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1987 Sep |
Date Detail:
|
Created Date: 1987-10-06 Completed Date: 1987-10-06 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 6030-4 Citation Subset: IM |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/M17373; M17374 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chickens Chimera Humans Interferon Type I / genetics* Muramidase / genetics Oocytes / metabolism Protein Biosynthesis* RNA, Messenger / genetics* Reticulocytes / metabolism Xenopus |
| Chemical | |
Reg. No./Substance:
|
0/Interferon Type I; 0/RNA, Messenger; EC 3.2.1.17/Muramidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro digestibility and amino acid composition of pearl millet (Pennisetum typhoides) and other c...
Next Document: Type XII collagen: distinct extracellular matrix component discovered by cDNA cloning.