Document Detail


The 3' end of large ribosomal subunit RNA from mosquito mitochondria: homogeneity of transcribed moieties.
MedLine Citation:
PMID:  2408289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
3' Terminal sequences of mosquito mitochondrial 16 S ribosomal RNA, which is post-transcriptionally polyadenylated, have been examined with the aim of determining the degree of homogeneity of transcribed moieties. 3' End-labeled samples were subjected to oligonucleotide fingerprint analysis and to ladder gel analysis after primary and secondary nuclease digestion; and products of reverse transcriptase reactions were characterized using 16 S RNA as template and selected oligodeoxynucleotides as primers. The results indicated a remarkable degree of homogeneity compared to homologous mammalian mitochondrial systems, and suggested differences in modes of expression of insect, versus mammalian, mitochondrial genomes.
Authors:
D T Dubin; C C HsuChen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Plasmid     Volume:  13     ISSN:  0147-619X     ISO Abbreviation:  Plasmid     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-07-02     Completed Date:  1985-07-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7802221     Medline TA:  Plasmid     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  139-44     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aedes / genetics*
Animals
Base Sequence
DNA / analysis,  genetics
Mitochondria / analysis*
Oligodeoxyribonucleotides / analysis,  genetics
RNA, Ribosomal / analysis*,  genetics
RNA-Directed DNA Polymerase
Templates, Genetic
Transcription, Genetic*
Grant Support
ID/Acronym/Agency:
GM 14957/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Oligodeoxyribonucleotides; 0/RNA, Ribosomal; 9007-49-2/DNA; EC 2.7.7.49/RNA-Directed DNA Polymerase

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