Document Detail


3-O-methyldobutamine, a major metabolite of dobutamine in humans.
MedLine Citation:
PMID:  11950781     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dobutamine is a synthetic ionotropic catecholamine commonly used to treat heart failure and shock. The catabolic fate of dobutamine in humans has yet to be reported, although formation of 3-O-methyldobutamine represents the principal pathway of dobutamine disposition in the dog. Herein, we describe the isolation and identification of 3-O-methyldobutamine in the urine of children receiving infusions of racemic dobutamine. In a 9-year-old child with heart failure approximately 80% of dobutamine administered intravenously at steady state was detected in the urine. Forty-seven percent of infused dobutamine was identified as 3-O-methyldobutamine and its acid-hydrolyzed derivatives, the latter mostly conjugated with sulfate (33%). Thirty-two percent consisted of acid-hydrolyzed dobutamine metabolites, primarily conjugated with sulfate (16%). Sonicates of human blood mononuclear cells catalyzed the formation of 3-O-methyldobutamine from dobutamine and S-adenosylmethionine in vitro. These findings indicate that formation of 3-O-methyldobutamine constitutes a major pathway of dobutamine metabolism in humans.
Authors:
Maohe Yan; Leslie T Webster; Jeffrey L Blumer
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Drug metabolism and disposition: the biological fate of chemicals     Volume:  30     ISSN:  0090-9556     ISO Abbreviation:  Drug Metab. Dispos.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-04-12     Completed Date:  2002-09-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421550     Medline TA:  Drug Metab Dispos     Country:  United States    
Other Details:
Languages:  eng     Pagination:  519-24     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio, USA.
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MeSH Terms
Descriptor/Qualifier:
Adrenergic beta-Agonists / metabolism*,  urine
Catechol O-Methyltransferase / metabolism
Child
Dobutamine / analogs & derivatives*,  metabolism*,  urine
Humans
Leukocytes, Mononuclear / enzymology,  metabolism
Magnetic Resonance Spectroscopy
Male
S-Adenosylmethionine / metabolism,  urine
Grant Support
ID/Acronym/Agency:
1 V10 HD 31313/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Adrenergic beta-Agonists; 29908-03-0/S-Adenosylmethionine; 34368-04-2/Dobutamine; 95231-13-3/3-O-methyldobutamine; EC 2.1.1.6/Catechol O-Methyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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