Document Detail


3-Methoxytyramine in the putamen as a gauge of the postmortem interval.
MedLine Citation:
PMID:  3734746     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The armamentarium of the forensic thanatologist includes the examiner's subjective assessment of bodily changes together with comprehensive evaluation of environmental and associated factors to estimate the postmortem interval (PMI). Of the various objective means, postmortem accumulation of potassium in vitreous humor is a widely used gauge. In view of the considerable variability inherent in these techniques, an additional marker with greater accuracy would be a welcome supplement to these indices. We have developed a method of estimating PMI from the level of 3-methoxytyramine (3-MT) in the dorsal putamen of the brain. The supernatant of a sample of putamen, sonicated in 6 volumes (weight/volume) of 0.1 M perchloric acid and centrifuged at 51 000 times gravity for 10 min, was analyzed for 3-MT using high-pressure liquid chromatography with electrochemical detection. The change of 3-MT in the putamen is linearly related to the PMI (range: 5.5 to 60 h), irrespective of patient age (1 to 84 years). This observation is attributable to several factors, including high substrate (dopamine) concentration, preservation of catechol-O-methyl transferase activity, and an inactivation of monoamine oxidase activity, as a result of a decrease in tissue pO2.
Authors:
D L Sparks; J T Slevin; J C Hunsaker
Related Documents :
1908106 - Deamination of 2-propyl-1-aminopentane and 2-[(2-propyl)pentylamino] acetamide by amine...
18838506 - Mechanism-based inactivation of cytochrome p450 2c9 by tienilic acid and (+/-)-suprofen...
6223456 - L-usnate-urease interactions: binding sites for polymerization.
15808886 - Downregulation of rac1 activation by caffeic acid in aortic smooth muscle cells.
6591186 - Identity of hepatic membrane transport systems for bile salts, phalloidin, and antamani...
22246806 - Saturated fatty acid induction of endoplasmic reticulum stress and apoptosis in human l...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of forensic sciences     Volume:  31     ISSN:  0022-1198     ISO Abbreviation:  J. Forensic Sci.     Publication Date:  1986 Jul 
Date Detail:
Created Date:  1986-09-25     Completed Date:  1986-09-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375370     Medline TA:  J Forensic Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  962-71     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Child
Child, Preschool
Chromatography, High Pressure Liquid
Dopamine / analogs & derivatives*,  analysis
Humans
Infant
Middle Aged
Postmortem Changes*
Putamen / analysis*
Time Factors
Grant Support
ID/Acronym/Agency:
RRO5374/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
554-52-9/3-methoxytyramine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Experimental study of postmortem change under field conditions: effects of freezing, thawing, and me...
Next Document:  Determination of age from the sternal rib in white females: a test of the phase method.