Document Detail


3-Hydroxypropionic acid and methylcitric acid are not reliable indicators of marginal biotin deficiency in humans.
MedLine Citation:
PMID:  14747666     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In two studies comprising 10 and 11 subjects, respectively, marginal biotin deficiency was induced experimentally by an egg-white diet in healthy men and women. The following urinary organic acids were assessed for their usefulness in detecting marginal biotin status: 1) 3-hydroxypropionic acid and methylcitric acid, organic acids that reflect decreased activity of the biotin-dependent enzyme propionyl-CoA carboxylase and 2) methylcrotonylglycine and isovalerylglycine, organic acids that reflect decreased activity of methylcrotonyl-CoA carboxylase. Mean 3-hydroxypropionic acid excretion rates remained normal during biotin depletion in both studies. By the end of the depletion period, 3-hydroxypropionic acid excretion identified only 5 of 21 marginally deficient subjects. Mean methylcitric acid excretion increased (P < 0.0001) in the first study but not in the second. Mean methylcrotonylglycine excretion increased in each study (P < 0.004 and P < 0.05, respectively); methylcrotonylglycine excretion identified 13 of 21 marginally deficient subjects. Mean isovalerylglycine excretion increased only in the first study (P = 0.006) and identified only 6 of 21 deficient subjects. We conclude that none of these organic acids is as sensitive an indicator of marginal biotin deficiency as 3-hydroxyisovaleric acid, which reflects decreased methylcrotonyl-CoA carboxylase.
Authors:
Donald M Mock; Cindy L Henrich-Shell; Nadine Carnell; Phyllis Stumbo; Nell I Mock
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of nutrition     Volume:  134     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-28     Completed Date:  2004-02-27     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  317-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biotin / administration & dosage,  deficiency*,  urine
Carbon-Carbon Ligases / metabolism,  urine
Citrates / diagnostic use*,  metabolism
Diet
Female
Gas Chromatography-Mass Spectrometry
Humans
Lactic Acid / analogs & derivatives*,  diagnostic use*,  metabolism
Male
Reference Values
Grant Support
ID/Acronym/Agency:
DK36823/DK/NIDDK NIH HHS; M01RR14288/RR/NCRR NIH HHS; R37 DK036823/DK/NIDDK NIH HHS; RR 00059/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Citrates; 33X04XA5AT/Lactic Acid; 6SO6U10H04/Biotin; C4ZF6XLD2X/hydracrylic acid; EC 6.4.-/Carbon-Carbon Ligases; EC 6.4.1.4/methylcrotonoyl-CoA carboxylase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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