| 3-D Model of the bee venom acid phosphatase: insights into allergenicity. | |
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MedLine Citation:
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PMID: 19059210 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The acid phosphatase Api m 3 is the major allergen of the honeybee venom. Except for the amino acid sequence, no other structural information for the enzyme is available. We applied homology modeling to assign the three-dimensional structure of Api m 3. The structure of the homodimeric human prostatic acid phosphatase was used to model the Api m 3 tertiary structure. IgE epitopes and antigenic sites were predicted using programs based on the structure of known epitopes and analysis of the 3-D model. The model of Api m 3 revealed an active site similar to those of the histidine-type acid phosphatases with conservation of the catalytically important residues. The observed substitutions in the phosphate ion binding site suggest differences in the substrate specificity in comparison to other acid phosphatases. The analysis of the Api m 3 three-dimensional model revealed a very likely mechanism of enzyme action. |
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Authors:
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Dessislava Georgieva; Kerstin Greunke; Nicolay Genov; Christian Betzel |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-04 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 378 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-30 Completed Date: 2009-01-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 711-5 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry and Molecular Biology, Hamburg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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chemistry*,
immunology Allergens / chemistry*, immunology Amino Acid Sequence Animals Bee Venoms / enzymology*, immunology Binding Sites Catalysis Crystallography, X-Ray Histidine / chemistry Humans Immunodominant Epitopes / chemistry*, immunology Models, Molecular Molecular Sequence Data Phosphates / chemistry Protein Structure, Tertiary Rats |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Bee Venoms; 0/Immunodominant Epitopes; 0/Phosphates; 71-00-1/Histidine; EC 3.1.3.2/Acid Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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