Document Detail

3-Chlorotyrosine as a marker of protein damage by myeloperoxidase in tracheal aspirates from preterm infants: association with adverse respiratory outcome.
MedLine Citation:
PMID:  12595594     Owner:  NLM     Status:  MEDLINE    
Oxidative injury is implicated in the development of chronic lung disease in preterm infants with respiratory distress. However, direct evidence of a causal role is limited and the source of reactive oxidants has not been identified. We have previously shown that protein carbonyl levels in tracheal aspirates correlate positively with myeloperoxidase, suggesting that neutrophil oxidants could be the source of this protein injury. We have extended these observations by measuring 3-chlorotyrosine, a specific biomarker of the neutrophil oxidant, hypochlorous acid, in tracheal aspirate proteins (144 samples) from 69 infants with birth weight <1500 g. 3-Chlorotyrosine levels were higher in these infants than in larger infants without respiratory distress (median 83 compared with 13 micromol/mol tyrosine). They correlated strongly with myeloperoxidase activity (correlation coefficient 0.75, p < 0.0001) and to a lesser extent with protein carbonyls. 3-Chlorotyrosine levels (at 1 wk after birth) correlated negatively with birth weight or gestational age. They were significantly higher in infants who developed chronic lung disease (oxygen requirement at 36 wk postmenstrual age) than in those who did not (median 88 and 49 micromol/mol tyrosine, respectively) and correlated with days of supplemental oxygen. 3-Chlorotyrosine was also significantly higher in infants who had lung infection or were Ureaplasma urealyticum positive. Our results are the first evidence that chlorinated proteins are produced in the lungs of premature infants and that they are higher in infection. The higher 3-chlorotyrosine levels in infants who develop chronic lung disease suggest that neutrophil oxidants contribute to the pathology of this disease.
I Hendrikje Buss; Revathy Senthilmohan; Brian A Darlow; Nina Mogridge; Anthony J Kettle; Christine C Winterbourn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric research     Volume:  53     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-02-21     Completed Date:  2003-08-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  455-62     Citation Subset:  IM    
Department of Pathology, Christchurch School of Medicine and Health Sciences, Christchurch, New Zealand.
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MeSH Terms
Biological Markers
Chronic Disease
Gestational Age
Infant, Newborn
Infant, Premature
Infant, Very Low Birth Weight
Neutrophils / metabolism
Oxidative Stress
Peroxidase / metabolism*
Respiratory Distress Syndrome, Newborn / metabolism*,  microbiology
Trachea / metabolism*
Tyrosine / analogs & derivatives*,  metabolism*
Ureaplasma Infections / metabolism
Ureaplasma urealyticum
Reg. No./Substance:
0/Biological Markers; 55520-40-6/Tyrosine; 7298-90-0/3-chlorotyrosine; EC
Erratum In:
Pediatr Res. 2003 May;53(5):868

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