Document Detail

2DE for proteome analysis of human metaphase chromosomes.
MedLine Citation:
PMID:  19381588     Owner:  NLM     Status:  MEDLINE    
Two-dimensional electrophoresis (2DE) is one of the most effective methods for the reliable separation of proteins in a single gel. In our proteome analyses of human chromosomes, we used two types of 2DE: two-dimensional isoelectric focusing SDS-polyacrylamide gel electrophoresis (2D IEF/SDS-PAGE) and radical-free and highly reduced two-dimensional polyacrylamide gel electrophoresis (RFHR 2D PAGE) together with one-dimensional SDS-polyacrylamide gel electrophoresis (1DE). Experimental details of these gel electrophoresis procedures that have been shown to be effective for human proteome analyses are described in detail.
Kiichi Fukui; Susumu Uchiyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  519     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-04-21     Completed Date:  2009-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-71     Citation Subset:  IM    
Department of Biotechnology, Laboratory of Dynamic Cell Biology, Graduate School of Engineering, Osaka University, Osaka, Japan.
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MeSH Terms
Cell Line
Chromosomes, Human / chemistry*
Electrophoresis, Gel, Two-Dimensional / methods*
Metaphase / genetics*
Proteome / analysis*
Reg. No./Substance:

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