| 2D separations on a 1D chip: gradient elution moving boundary electrophoresis-chiral capillary zone electrophoresis. | |
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MedLine Citation:
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PMID: 20886128 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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A new method is described for two-dimensional (2D) separations using a microfluidic chip normally employed for single dimension electrophoresis. The method employs a combination of gradient elution moving boundary electrophoresis (GEMBE) and chiral capillary zone electrophoresis (CZE). The simplicity of the first dimension GEMBE method enables its implementation in the injection channel of a conventional electrophoresis chip, simplifying the design and operation of the device. The method was used for high resolution 2D chiral separations of a mixture of amino acids considered as possible signatures of extant or extinct life for solar system exploration. The enantiomers of aspartic acid, glutamic acid, serine, alanine, and valine were all resolved as well as glycine (achiral) and several unidentified impurities, giving an estimated peak capacity of 35 for the region between valine and glycine. The results highlight the need for high peak capacity separations for chiral amino acid analysis if accurate enantiomeric ratios are to be determined. |
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Authors:
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David Ross; Jonathan G Shackman; Jason G Kralj; Javier Atencia |
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Publication Detail:
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Type: Journal Article Date: 2010-09-30 |
Journal Detail:
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Title: Lab on a chip Volume: 10 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-27 Completed Date: 2011-01-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 3139-48 Citation Subset: - |
Affiliation:
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National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. david.ross@nist.gov |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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