Document Detail


2D label-free imaging of resonant grating biochips in ultraviolet.
MedLine Citation:
PMID:  20589008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
2D images of label-free biochips exploiting resonant waveguide grating (RWG) are presented. They indicate sensitivities on the order of 1 pg/mm2 for proteins in air, and hence 10 pg/mm2 in water can be safely expected. A 320x256 pixels Aluminum-Gallium-Nitride-based sensor array is used, with an intrinsic narrow spectral window centered at 280 nm. The additional role of characteristic biological layer absorption at this wavelength is calculated, and regimes revealing its impact are discussed. Experimentally, the resonance of a chip coated with protein is revealed and the sensitivity evaluated through angular spectroscopy and imaging. In addition to a sensitivity similar to surface plasmon resonance (SPR), the RWGs resonance can be flexibly tailored to gain spatial, biochemical, or spectral sensitivity.
Authors:
K Bougot-Robin; J-L Reverchon; M Fromant; L Mugherli; P Plateau; H Benisty
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Optics express     Volume:  18     ISSN:  1094-4087     ISO Abbreviation:  Opt Express     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-06-30     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137103     Medline TA:  Opt Express     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11472-82     Citation Subset:  IM    
Affiliation:
Thales Research & Technology, 1 avenue Fresnel, Campus Polytechnique, 91767 Palaiseau, France. kristelle_robin@yahoo.fr
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / instrumentation*
Equipment Design
Equipment Failure Analysis
Protein Array Analysis / instrumentation*
Refractometry / instrumentation*
Staining and Labeling
Surface Plasmon Resonance / instrumentation*
Transducers*
Ultraviolet Rays

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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