| 27-Hydroxycholesterol causes remodeling in endothelial cell membrane lipid composition comparable to remodeling in the failed vein grafts of CABG patients. | |
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MedLine Citation:
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PMID: 16154158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our objective is to determine if vascular remodeling in CABG patients is related to oxysterols, therefore, we compared failed vein grafts from 18 patients, available after a second coronary artery bypass grafting (CABG), with human endothelial cells (ECs). The ECs were cultured in minimum essential medium (MEM) with or without 27-hydroxycholesterol (27OHC), one of the oxysterol products of oxidatively modified low density lipoproteins (ox-LDL), as an agent to alter molecular mechanisms in vascular cells. Significant changes in phospholipid composition, in fatty acid profile and in calcium concentration were found in the failed vein compared to the native saphenous vein from the same (CABG) patient. The failed vein contained significantly less phosphatidylethanolamine, more sphingomyelin, less arachidonic acid, more linoleic acid and more calcium than the native saphenous vein. Comparable changes in phospholipid composition, in fatty acid profile and increased calcium influx were reproduced in ECs cultured in medium containing 27OHC indicating that an oxysterol is an agent that can alter the lipid composition of vascular cell membranes. Our study indicates that a lipid agent, as well as protein agents that have previously been linked to the process of vascular remodeling, may be fundamental to many vascular diseases. |
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Authors:
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Fred A Kummerow; Mohamedain M Mahfouz; Qi Zhou; L Scott Cook |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-09 |
Journal Detail:
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Title: Life sciences Volume: 78 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-23 Completed Date: 2006-02-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: England |
Other Details:
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Languages: eng Pagination: 958-63 Citation Subset: IM |
Affiliation:
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Burnsides Research Laboratory, University of Illinois, 1208 W. Pennsylvania Ave., Urbana IL 61801, USA. fkummero@uiuc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcium
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metabolism Calcium Radioisotopes / diagnostic use Cell Membrane / drug effects, metabolism Cells, Cultured Coronary Artery Bypass* Endothelial Cells / drug effects, metabolism* Humans Hydroxycholesterols / pharmacology* Linoleic Acid / metabolism Lipid Metabolism / drug effects* Phospholipids / metabolism Phosphorus / metabolism Treatment Failure Veins / transplantation* |
| Chemical | |
Reg. No./Substance:
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0/Calcium Radioisotopes; 0/Hydroxycholesterols; 0/Phospholipids; 20380-11-4/27-hydroxycholesterol; 2197-37-7/Linoleic Acid; 7440-70-2/Calcium; 7723-14-0/Phosphorus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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