Document Detail

A 2.5-kb insert eliminates acid soluble invertase isozyme II transcript in carrot (Daucus carota L.) roots, causing high sucrose accumulation.
MedLine Citation:
PMID:  14756313     Owner:  NLM     Status:  MEDLINE    
The predominant storage carbohydrates of mature carrot (Daucus carota L.) storage roots typically are the free sugars glucose and fructose. This trait is conditioned by the Rs allele. A naturally occurring recessive mutation, rs/rs, conditions a shift from these reducing sugars to sucrose. RT-PCR and sequencing revealed a unique 2.5 kb insert in the first and largest intron near the 5' end of the acid soluble invertase isozyme II gene of rs/rs carrots. This insert was not totally spliced out during mRNA processing. While the wild-type acid-soluble invertase isozyme II transcript (ca. 2 kb) was detected in Rs/Rs roots and leaves, none was observed in rs/rs roots throughout development. RT-PCR of rs/rs leaves revealed two novel transcripts (2.7 kb and 3.2 kb). A comparison of enzyme activity between the near-isogenic Rs/Rs and rs/rs carrot lines revealed very low acid-soluble invertase activity in rs/rs roots whereas neutral invertase, sucrose synthase and sucrose phosphate synthase levels were comparable. Those results and linkage analysis indicate that Rs is a candidate locus for carrot vacuolar acid-soluble invertase isozyme II. Although the 2.5 kb insert does not occur in the Rs wild-type acid-soluble invertase isozyme II allele, it does occur elsewhere in the genome of Rs/Rs plants.
Yuan-Yeu Yau; Philipp W Simon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant molecular biology     Volume:  53     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2004-02-03     Completed Date:  2004-04-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  151-62     Citation Subset:  IM    
USDA-ARS Vegetable Research Crops Unit, Department of Horticulture, University of Wisconsin-Madison, 1575 Leiden Drive, Madison, WI 53706, USA.
Data Bank Information
Bank Name/Acc. No.:
PIR/AY368232;  AY368233;  AY368234
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MeSH Terms
DNA, Complementary / chemistry,  genetics
DNA, Plant / chemistry,  genetics
Daucus carota / enzymology,  genetics*,  metabolism
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Isoenzymes / genetics
Molecular Sequence Data
Mutagenesis, Insertional*
Plant Leaves / enzymology,  genetics
Plant Roots / enzymology,  genetics*,  metabolism
RNA, Plant / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Sucrose / metabolism*
Time Factors
Transcription, Genetic
beta-Fructofuranosidase / genetics*
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Plant; 0/Isoenzymes; 0/RNA, Plant; 57-50-1/Sucrose; EC

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