Document Detail


25-Hydroxycholesterol causes death but does not prevent nerve growth factor-induced neurite outgrowth in PC12 cells.
MedLine Citation:
PMID:  9308000     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of cholesterol oxides on regular and neuronal PC12 cells were examined. Among the cholesterol oxides tested, 7-beta-, 22(R)- and 25-OH-cholesterol were very toxic to regular PC12 cells such that approximately 80% of cells were killed after a 3 day incubation with 20 micrograms/ml (50 microM) of these agents. Cholesterol oxides caused cytotoxicity in neuronal PC12 cells at concentrations higher than those observed for regular PC12 cells. The nerve growth factor (NGF)-induced neurite outgrowth and nitric oxide synthase expression appeared normal in PC12 cells treated with a sublethal dose of 25-OH-cholesterol. Neurite regeneration from established neuronal PC12 cells was not inhibited by the presence of a toxic dose of 25-OH-cholesterol. Given the fact that 25-OH-cholesterol is a potent inhibitor of cholesterol synthesis pathway, these results suggest that the de novo synthesis of cholesterol may not be required for NGF-induced neurite outgrowth in PC12 cells.
Authors:
J Y Chang; L Z Liu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemistry international     Volume:  31     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1998-01-06     Completed Date:  1998-01-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  517-23     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Death
Cell Differentiation
Cholesterol / pharmacology
Dose-Response Relationship, Drug
Hydroxycholesterols / pharmacology*,  poisoning
Nerve Growth Factors / pharmacology*
Nerve Regeneration
Neurites / drug effects*,  physiology*
Neurons / drug effects
Oxides / pharmacology
PC12 Cells / drug effects,  pathology,  physiology
Rats
Grant Support
ID/Acronym/Agency:
NS32253/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Hydroxycholesterols; 0/Nerve Growth Factors; 0/Oxides; 2140-46-7/25-hydroxycholesterol; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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