Document Detail

24-hour preservation of isolated rat hearts perfused with pyridoxalated hemoglobin polyoxyethylene conjugate (PHP) solution at low temperature.
MedLine Citation:
PMID:  2036066     Owner:  NLM     Status:  MEDLINE    
We examined the effects of long-term perfusion with pyridoxalated hemoglobin polyoxyethylene conjugate (PHP) solution on cardiac function of isolated rat hearts. Hearts were perfused with oxygenated Krebs-Henseleit (K-H) solution containing 3% PHP or hydroxyethyl starch (HES) at a constant pressure of 13 mm Hg for 24 h at 15 degrees C. After 24 h preservation, hearts were rewarmed with K-H solution. Heart rate (HR) in PHP-preserved hearts was almost the same as control and cardiac contractility was maintained at 70% of control, but coronary outflow decreased to about 50% of control. No edema developed in the PHP group. Addition of a Ca2+ antagonist, diltiazem, inhibited the elevation of the end-diastolic pressure significantly. In HES-preserved hearts, HR did not recover to control levels, but there was no significant difference in cardiac contractility between PHP- and HES-perfused hearts. The results demonstrate that isolated hearts can be preserved by perfusion with PHP solution under hypothermic conditions for 24 h and suggest that PHP solution would be useful for a perfusate of isolated organs and tissues for preservation.
S Iwasaki; H Araki; Y Miyauchi; K Nishi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Artificial organs     Volume:  15     ISSN:  0160-564X     ISO Abbreviation:  Artif Organs     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-06-24     Completed Date:  1991-06-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  78-85     Citation Subset:  IM    
Department of Pharmacology, Kumamoto University Medical School, Japan.
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MeSH Terms
Blood Substitutes*
Heart Rate / physiology
Hypothermia, Induced
Myocardial Contraction / physiology
Myocardial Reperfusion
Organ Preservation*
Polyethylene Glycols*
Rats, Inbred Strains
Time Factors
Reg. No./Substance:
0/Blood Substitutes; 0/Hemoglobins; 0/Polyethylene Glycols; 0/pyridoxalated-hemoglobin-polyethylene glycol conjugate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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