Document Detail


24 Hours in the Life of HIV-1 in a T Cell Line.
MedLine Citation:
PMID:  23382686     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
HIV-1 infects CD4+ T cells and completes its replication cycle in approximately 24 hours. We employed repeated measurements in a standardized cell system and rigorous mathematical modeling to characterize the emergence of the viral replication intermediates and their impact on the cellular transcriptional response with high temporal resolution. We observed 7,991 (73%) of the 10,958 expressed genes to be modulated in concordance with key steps of viral replication. Fifty-two percent of the overall variability in the host transcriptome was explained by linear regression on the viral life cycle. This profound perturbation of cellular physiology was investigated in the light of several regulatory mechanisms, including transcription factors, miRNAs, host-pathogen interaction, and proviral integration. Key features were validated in primary CD4+ T cells, and with viral constructs using alternative entry strategies. We propose a model of early massive cellular shutdown and progressive upregulation of the cellular machinery to complete the viral life cycle.
Authors:
Pejman Mohammadi; Sébastien Desfarges; István Bartha; Beda Joos; Nadine Zangger; Miguel Muñoz; Huldrych F Günthard; Niko Beerenwinkel; Amalio Telenti; Angela Ciuffi
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Publication Detail:
Type:  Journal Article     Date:  2013-01-31
Journal Detail:
Title:  PLoS pathogens     Volume:  9     ISSN:  1553-7374     ISO Abbreviation:  PLoS Pathog.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-02-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101238921     Medline TA:  PLoS Pathog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1003161     Citation Subset:  IM    
Affiliation:
Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland ; SIB Swiss Institute of Bioinformatics, Basel and Lausanne, Switzerland.
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