| 2,3,7,8-Tetrachlorodibenzo-p-dioxin modifies expression and nuclear/cytosolic localization of bovine herpesvirus 1 immediate-early protein (bICP0) during infection. | |
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MedLine Citation:
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PMID: 20506271 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our previous studies have demonstrated that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) increases Bovine Herpesvirus 1 (BHV-1) replication through a dose-dependent increase in cytopathy and increased viral titer. Furthermore, TCDD was able to trigger BHV-1-induced apoptosis by up-regulating the activation of initiator caspases 8 and 9, as well as of effector caspase 3. Since TCDD activates caspase 3 after 4 h of infection, we have hypothesized an involvement of BHV-1 infected cell protein 0 (bICP0) in this process. Such protein, the major transcriptional regulatory protein of BHV-1, has been shown to indirectly induce caspase 3 activation and apoptosis. In order to elucidate the role of bICP0 in this apoptotic pathway, here we have analyzed the effects of TCDD on bICP0 expression. Following infection of bovine cells with BHV-1, we detected apoptotic features already at 12 h after infection, only in TCDD exposed groups. Furthermore, in the presence of different doses of TCDD, we observed a time-dependent modulation and increase of bICP0 gene expression levels, as revealed by RT-PCR analysis. Western blot analysis and immunocytochemistry revealed that TCDD induced an increase of bICP0 protein levels in a dose-dependent manner, compared to unexposed groups. Moreover, Western blot analysis of nuclear and cytosolic fractions of infected cells revealed that TCDD anticipated the presence of bICP0 protein in the cytoplasm. In conclusion, both the increase of replication of BHV-1 and anticipation of BHV-1-induced apoptosis could be the result of a relationship between TCDD and bICP0. |
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Authors:
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Filomena Fiorito; Gabriella Marfè; Giovanna E Granato; Roberto Ciarcia; Emma De Blasio; Marco Tafani; Salvatore Florio; Luisa De Martino; Gianmarco Muzi; Ugo Pagnini; Antonio Giordano |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 111 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-27 Completed Date: 2011-01-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 333-42 Citation Subset: IM |
Copyright Information:
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© 2010 Wiley-Liss, Inc. |
Affiliation:
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Department of Pathology and Animal Health, University of Naples Federico II, Naples, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Active Transport, Cell Nucleus* Animals Apoptosis Cattle Cytosol Gene Expression Regulation, Viral / drug effects* Herpesviridae Infections Herpesvirus 1, Bovine / chemistry, physiology Immediate-Early Proteins / genetics Tetrachlorodibenzodioxin / pharmacology* Trans-Activators / genetics*, metabolism* Ubiquitin-Protein Ligases / genetics*, metabolism* Viral Proteins / genetics, metabolism Virus Replication |
| Chemical | |
Reg. No./Substance:
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0/Immediate-Early Proteins; 0/Trans-Activators; 0/Viral Proteins; 1746-01-6/Tetrachlorodibenzodioxin; EC 6.3.2.19/Ubiquitin-Protein Ligases; EC 6.3.2.19/bICP0 protein, Bovine herpesvirus 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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