| A 210 kDa nuclear matrix protein is a functional part of the mitotic spindle; a microinjection study using SPN monoclonal antibodies. | |
| | |
MedLine Citation:
|
PMID: 1915296 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Six monoclonal antibodies identify a 210 kDa polypeptide which shows a cell cycle specific redistribution from the nucleus to the mitotic spindle. In interphase cells this polypeptide was localized in the nucleus and behaved during differential cell extraction as a component of the nuclear matrix. It accumulated in the centrosome region at prophase, in the pole regions of the mitotic spindle at metaphase and in crescents at the poles in anaphase, and reassociated with the nuclei as they reformed in telophase. Due to its staining pattern we call the protein the Spindle Pole-Nucleus (SPN) antigen. The localization of SPN antigen during mitosis was dependent on the integrity of the spindle since treatment of cells with nocodazole resulted in the dispersal of SPN antigen into many small foci which acted as microtubule organizing centres when the drug was removed. The SPN antigen was present in nuclei and mitotic spindles of all human and mammalian cell lines and tissues so far tested. When microinjected into the cytoplasm or nuclei of HeLa cells, one antibody caused a block in mitosis. Total cell number remained constant or decreased slightly after 24 h. At this time, about half the cells were arrested in a prometaphase-like state and revealed aberrant spindles. Many other cells were multinucleate. These results show that the SPN antigen is a protein associated with mitotic spindle microtubules which has to function correctly for the cell to complete mitosis. |
| | |
Authors:
|
M Kallajoki; K Weber; M Osborn |
Related Documents
:
|
20719276 - Assessment of mitotic spindle phenotypes in drosophila s2 cells. 19223556 - Novel microtubule polymerization inhibitor with potent antiproliferative and antitumor ... 2161766 - Mitosis arrested by deuterium oxide. light microscopic, immunofluorescence and ultrastr... 12937986 - The association of peroxisomes with the developing cell plate in dividing onion root ce... 10854556 - Inhibitory effect of resveratrol on the proliferation of human and rat hepatic derived ... 2549976 - Low-density-lipoprotein receptors in different rabbit liver cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The EMBO journal Volume: 10 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 1991 Nov |
Date Detail:
|
Created Date: 1991-11-18 Completed Date: 1991-11-18 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 8208664 Medline TA: EMBO J Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 3351-62 Citation Subset: IM |
Affiliation:
|
Max Planck Institute for Biophysical Chemistry, Department of Biochemistry, Göttingen, FRG. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antibodies, Monoclonal
/
diagnostic use* Antigens, Nuclear Autoantigens / immunology Blotting, Western Fluorescent Antibody Technique Hela Cells Humans Immunoglobulin G / immunology Microinjections Mitosis Mitotic Spindle Apparatus / chemistry*, drug effects Molecular Weight Nocodazole / pharmacology Nuclear Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Antigens, Nuclear; 0/Autoantigens; 0/Immunoglobulin G; 0/Nuclear Proteins; 31430-18-9/Nocodazole |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Plastid translation and transcription genes in a non-photosynthetic plant: intact, missing and pseud...
Next Document: A factor that positively regulates cell division by activating transcription of the major cluster of...