Document Detail


20-hydroxyeicosatetraenoic acid (20-HETE): structural determinants for renal vasoconstriction.
MedLine Citation:
PMID:  12788354     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of natural and synthetic eicosanoids on the diameter of rat interlobular arteries studied in vitro were compared to that of the potent, endogenous vasoconstrictor 20-HETE. Vasoconstrictor activity was optimum for chain lengths of 20-22 carbons with at least one olefin or epoxide between located between C(13)-C(15) and an oxygen substituent at C(20)-C(22). The presence of delta (Zou et al. Am. J. Physiol. 1996, 270, R228; Gebremedhin, D. et al. Am. J. Physiol. 1998, 507, 771)-, delta (Carroll et al. Am. J. Physiol. 1996, 271, R863; Vazquez et al. Life Sci. 1995, 56, 1455)-, or delta (Imig et al. Hypertension 2000, 35, 307; Lopez et al. Amer. J. Physiol. 2001, 281, F420)-olefins had no influence on the vasoconstrictor response whereas the introduction of a C(7)-thiomethylene enhanced potency. A sulfonamide or alcohol, but not a lactone, could replace the C(1)-carboxylate. These data were used to construct a putative binding domain map of the 20-HETE receptor consisting of: (i) a comparatively open, hydrophilic binding site accommodating the C(1)-functionality; (ii) a hydrophobic trough spanning the olefins; (iii) a shallow pocket containing a critical pi-pi binding site in the vicinity of the pi (Ito et al. Am. J. Physiol. 1998, 274, F395; Quigley, R.; Baum, M.; Reddy, K. M.; Griener, J. C.; Falck, J. R. Am. J. Physiol. 2000, 278, F949)-olefin; and (iv) an oxyphilic binding site proximate to the omega-terminus.
Authors:
Ming Yu; Magdalena Alonso-Galicia; Cheng-Wen Sun; Richard J Roman; Naoya Ono; Hitomi Hirano; Tsuyoshi Ishimoto; Y Krishna Reddy; Kishta Reddy Katipally; Komandla Malla Reddy; V Raj Gopal; Ji Yu; Mohamed Takhi; J R Falck
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Bioorganic & medicinal chemistry     Volume:  11     ISSN:  0968-0896     ISO Abbreviation:  Bioorg. Med. Chem.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-05     Completed Date:  2004-04-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9413298     Medline TA:  Bioorg Med Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  2803-21     Citation Subset:  IM    
Affiliation:
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Hydroxyeicosatetraenoic Acids / chemical synthesis*,  pharmacology*
Kidney / blood supply
Magnetic Resonance Spectroscopy
Rats
Receptors, Eicosanoid / chemistry
Structure-Activity Relationship
Vasoconstriction / drug effects*
Grant Support
ID/Acronym/Agency:
DK38226/DK/NIDDK NIH HHS; GM 31287/GM/NIGMS NIH HHS; HL29587/HL/NHLBI NIH HHS; HL36279/HL/NHLBI NIH HHS; HL59996/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Hydroxyeicosatetraenoic Acids; 0/Receptors, Eicosanoid; 79551-86-3/20-hydroxy-5,8,11,14-eicosatetraenoic acid

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