Document Detail

A 20-amino acid module of protein kinase C{epsilon} involved in translocation and selective targeting at cell-cell contacts.
MedLine Citation:
PMID:  19429675     Owner:  NLM     Status:  MEDLINE    
In the pituitary gland, activated protein kinase C (PKC) isoforms accumulate either selectively at the cell-cell contact (alpha and epsilon) or at the entire plasma membrane (beta1 and delta). The molecular mechanisms underlying these various subcellular locations are not known. Here, we demonstrate the existence within PKCepsilon of a cell-cell contact targeting sequence (3CTS) that, upon stimulation, is capable of targeting PKCdelta, chimerin-alpha1, and the PKCepsilon C1 domain to the cell-cell contact. We show that this selective targeting of PKCepsilon is lost upon overexpression of 3CTS fused to a (R-Ahx-R)(4) (where Ahx is 6-aminohexanoic acid) vectorization peptide, reflecting a dominant-negative effect of the overexpressed 3CTS on targeting selectivity. 3CTS contains a putative amphipathic alpha-helix, a 14-3-3-binding site, and the Glu-374 amino acid, involved in targeting selectivity. We show that the integrity of the alpha-helix is important for translocation but that 14-3-3 is not involved in targeting selectivity. However, PKCepsilon translocation is increased when PKCepsilon/14-3-3 interaction is abolished, suggesting that phorbol 12-myristate 13-acetate activation may initiate two sets of PKCepsilon functions, those depending on 14-3-3 and those depending on translocation to cell-cell contacts. Thus, 3CTS is involved in the modulation of translocation via its 14-3-3-binding site, in cytoplasmic desequestration via the alpha-helix, and in selective PKCepsilon targeting at the cell-cell contact via Glu-374.
Barthélémy Diouf; Alejandra Collazos; Gilles Labesse; Françoise Macari; Armelle Choquet; Philippe Clair; Cécile Gauthier-Rouvière; Nathalie C Guérineau; Philippe Jay; Frédéric Hollande; Dominique Joubert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-08
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  284     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-06     Completed Date:  2009-09-01     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18808-15     Citation Subset:  IM    
CNRS UMR5203, INSERM, U661, University of Montpellier I and II, France.
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MeSH Terms
14-3-3 Proteins / chemistry
Amino Acid Sequence
Amino Acids / chemistry
Binding Sites
Cell Communication
Glutamic Acid / chemistry
Molecular Sequence Data
Pituitary Gland / metabolism
Protein Conformation
Protein Kinase C-epsilon / chemistry,  physiology*
Protein Structure, Secondary
Protein Structure, Tertiary
Protein Transport
Tetradecanoylphorbol Acetate / chemistry
Reg. No./Substance:
0/14-3-3 Proteins; 0/Amino Acids; 16561-29-8/Tetradecanoylphorbol Acetate; 56-86-0/Glutamic Acid; EC Kinase C-epsilon

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