Document Detail

2-NBDG, a Fluorescent Analogue of Glucose, as a Marker for Detecting Cell Electropermeabilization In Vitro.
MedLine Citation:
PMID:  22886206     Owner:  NLM     Status:  Publisher    
This study investigated whether molecules spontaneously transported inside cells, like glucose derivatives, can also be used as electropermeabilization markers. Uptake of a fluorescent deoxyglucose derivative (2-NBDG) by normal and electropermeabilized cells in culture was analyzed. 2-NBDG was added to DC-3F cell suspensions and cells, exposed or not to eight square-wave electric pulses of 100-μs duration and of appropriate field amplitude at a repetition frequency of 1 Hz or 5 kHz, were incubated at 37 °C. 2-NBDG uptake was temperature-, concentration- and time-dependent in cells submitted or not to the electric pulses. In spite of significant uptake of 2-NBDG mediated by GLUT transporters into nonpermeabilized cells, the electric pulses significantly increased about ten to hundred times the 2-NBDG uptake into the cells. The increase in the field amplitude from 900 to 1,500 V/cm resulted in a progressive increase of 2-NDBG. Our results show that under the conditions of in vivo exposure duration to FDG and the physiological concentration of D: -glucose, electric pulses increased 2-NBDG uptake into electropermeabilized cells. Under our experimental conditions, the percentage of permeabilized cells within the population of cells exposed to electric pulses remained at the same level regardless of the pulse frequency used, 1 Hz or 5 kHz. The findings showed that glucose derivatives can also be used to detect electropermeabilized cells exposed to electric pulses.
Elham Raeisi; Lluis M Mir
Related Documents :
1450106 - Steady discharges of x and y retinal ganglion cells of cat under photopic illuminance.
527646 - Paradoxical gaba excitation of nigral dopaminergic cells: indirect mediation through re...
21804216 - The β-carboline alkaloid harmol induces cell death via autophagy but not apoptosis in ...
22266826 - Comparison of aldosterone production among human adrenocortical cell lines.
20224986 - Establishment and characterization of multi-drug resistant, prostate carcinoma-initiati...
1915296 - A 210 kda nuclear matrix protein is a functional part of the mitotic spindle; a microin...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-11
Journal Detail:
Title:  The Journal of membrane biology     Volume:  -     ISSN:  1432-1424     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Alkyne-coordinating tridentate ligands: structural properties and reactivity of their rhodium comple...
Next Document:  Molecular-Level Characterization of Lipid Membrane Electroporation using Linearly Rising Current.