Document Detail


2-Methoxyestradiol induces cell cycle arrest and apoptosis of nasopharyngeal carcinoma cells.
MedLine Citation:
PMID:  15525476     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate 2-methoxyestradiol induced apoptosis and its mechanism of action in CNE2 cell lines. METHODS: CNE2 cells were cultured in RPMI-1640 medium and treated with 2-methoxyestradiol in different concentrations. MTT assay was used to detect growth inhibition. Flow cytometry and DNA ladders were used to detect apoptosis. Western blotting was used to observe the expression of p53, p21(WAF1), Bax, and Bcl-2 protein. RESULTS: 2-methoxyestradiol inhibited proliferation of nasopharyngeal carcinoma CNE2 cells with IC(50) value of 2.82 micromol/L. The results of flow cytometry showed an accumulation of CNE2 cells in G2/M phase in response to 2-methoxyestradiol. Treatment of CNE2 cells with 2-methoxyestradiol resulted in DNA fragmentation. The expression levels of protein p53 and Bcl-2 decreased following 2-methoxyestradiol treatment in CNE2 cells, whereas Bax and p21(WAF1) protein expression were unaffected after treatment with 2-methoxyestradiol. CONCLUSION: These results suggest that 2-methoxyestradiol induced cell cycle arrest at G2/M phase and apoptosis of CNE2 cells which was associated to Bcl-2 down-regulation.
Authors:
Ning-ning Zhou; Xiao-feng Zhu; Jun-ming Zhou; Man-zhi Li; Xiao-shi Zhang; Peng Huang; Wen-qi Jiang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  25     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-04     Completed Date:  2005-03-18     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  1515-20     Citation Subset:  IM    
Affiliation:
Cancer Center, Sun Yat-sen University, Guangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Hormonal / administration & dosage,  pharmacology*
Apoptosis / drug effects*
Cell Cycle / drug effects
Cell Line, Tumor
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Down-Regulation
Estradiol / administration & dosage,  analogs & derivatives*,  pharmacology*
Humans
Nasopharyngeal Neoplasms / metabolism,  pathology*
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Tumor Suppressor Protein p53 / metabolism
bcl-2-Associated X Protein
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Hormonal; 0/BAX protein, human; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 0/bcl-2-Associated X Protein; 362-07-2/2-methoxyestradiol; 50-28-2/Estradiol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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