| 2'-Epi-2'-O-acetylthevetin B induces apoptosis partly via Ca(2+)-mediated mitochondrial pathway in human hepatocellular carcinoma HepG2 cells. | |
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MedLine Citation:
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PMID: 19524689 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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2'-Epi-2'-O-acetylthevetin B (GHSC-74), a cardiac glycoside, can be isolated from the seeds of Cerbera manghas L. We demonstrated that GHSC-74 reduced the viability of HepG2 cells in a time- and dose-dependent manner, and efficiently induced apoptosis without significantly decreasing the viability of Chang human liver cells and Swiss albino 3T3 fibroblasts, as indicated by annexin-V/PI binding assay and Hoechst 33342 staining. In addition, stimulation of HepG2 cells with GHSC-74 induced a series of intracellular events: (1) loss of mitochondrial membrane potential; (2) sustained elevation of cytosolic [Ca2+]; and (3) downregulation of Bcl-2. BAPTA-AM, a cytosolic Ca2+ chelator, partly suppressed cell death and prevented mitochondrial membrane potential from losing in GHSC-74-treated HepG2 cells. In contrast, EGTA, an extracellular Ca2+ chelator, exhibited a weaker effect as compared to that of BAPTA-AM. Taken together, the Ca(2+)-mediated mitochondrial pathway was found to be involved in GHSC-74-induced HepG2 cell apoptosis. |
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Authors:
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Bo Feng; Cai-Guo Huang; Ruo-Hua Chen; Yue-Wei Guo; Bing-Hua Jiao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-12 |
Journal Detail:
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Title: Cell biology international Volume: 33 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-31 Completed Date: 2009-12-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: Netherlands |
Other Details:
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Languages: eng Pagination: 918-25 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Anticarcinogenic Agents / chemistry, pharmacology* Apocynaceae / chemistry Apoptosis* Calcium / metabolism Cardiac Glycosides / chemistry, pharmacology* Cell Line, Tumor Chelating Agents / pharmacology Egtazic Acid / analogs & derivatives, pharmacology Enzyme Inhibitors / chemistry, pharmacology* Fibroblasts / drug effects Membrane Potential, Mitochondrial / drug effects, physiology Mice Mitochondria / drug effects, metabolism Proto-Oncogene Proteins c-bcl-2 / antagonists & inhibitors, metabolism* Signal Transduction / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
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0/2'-epi-2'-O-acetylthevetin B; 0/Anticarcinogenic Agents; 0/Cardiac Glycosides; 0/Chelating Agents; 0/Enzyme Inhibitors; 0/Proto-Oncogene Proteins c-bcl-2; 139890-68-9/1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; 67-42-5/Egtazic Acid; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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