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2'-Deoxy-2'-fluorouridine-5'-phosphate: an alternative substrate for thymidylate synthetase from Escherichia coli K12.
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MedLine Citation:
PMID:  370779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The substrate specificity of 2'-deoxy-2'-substituted uridines and their 5'-phosphates towards thymidylate synthetase from Escherichia coli K12 was investigated. Besides the natural substrate 2'-deoxyuridine-5'-phosphate (dUMP), only 2'-deoxy-2'-fluorouridine-5'-phosphate (dUflMP) was a substrate. The KM of dUflMP is 11 times higher than that of dUMP, while the Vmax values are virtually the same. It is concluded that the size of the 2'-substituent and not its polarity (and the concomitant conformational change) determines substrate specificity of thymidylate synthetase.
Authors:
F Wohlrab; T Haertlé; T Trichtinger; W Guschlbauer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleic acids research     Volume:  5     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1978 Dec 
Date Detail:
Created Date:  1979-05-16     Completed Date:  1979-05-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4753-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Deoxyuracil Nucleotides*
Escherichia coli / enzymology*
Fluorodeoxyuridylate*
Kinetics
Methyltransferases / metabolism*
Molecular Conformation
Substrate Specificity
Thymidylate Synthase / metabolism*
Chemical
Reg. No./Substance:
0/Deoxyuracil Nucleotides; 134-46-3/Fluorodeoxyuridylate; EC 2.1.1.-/Methyltransferases; EC 2.1.1.45/Thymidylate Synthase
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Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Month: 12 Year: 1978
Volume: 5 Issue: 12
First Page: 4753 Last Page: 4759
ID: 342786
PubMed Id: 370779

2'-Deoxy-2'-fluorouridine-5'-phosphate: an alternative substrate for thymidylate synthetase from Escherichia coli K12.
F Wohlrab
T Haertl?
T Trichtinger
W Guschlbauer



Article Categories:
  • Research Article


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