| 2-Deoxy-D-Glucose Attenuates Isoflurane-Induced Cytotoxicity in an In Vitro Cell Culture Model of H4 Human Neuroglioma Cells. | |
| | |
MedLine Citation:
|
PMID: 21965367 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Background:β-Amyloid protein (Aβ) accumulation and caspase activation have been shown to contribute to Alzheimer disease neuropathogenesis. Aβ is produced from amyloid precursor protein through proteolytic processing by aspartyl protease β-site amyloid precursor protein- cleaving enzyme (BACE). The inhaled anesthetic isoflurane has been shown to induce caspase activation and increase levels of BACE and Aβ. However, the underlying mechanisms and interventions of the isoflurane-induced neurotoxicity remain largely to be determined. The glucose analog 2-deoxy-d-glucose (2-DG) has neuroprotective effects. Therefore, we sought to determine whether 2-DG can reduce caspase-3 activation and the increase in the levels of BACE and reactive oxygen species (ROS) induced by isoflurane.Methods:H4 human neuroglioma cells were treated with saline or 2-DG (5 mM) for 1 hour followed by a control condition or 2% isoflurane for 6 hours. The levels of caspase-3 cleavage (activation), BACE, cytosolic calcium, and ROS were determined. Two-way analysis of variance was used to assess the interactions of 2-DG and isoflurane on caspase-3 activation, and levels of BACE and ROS.Results:In H4 human neuroglioma cells, 2-DG reduced the caspase-3 activation (477% vs 186%, F = 8.68; P = 0.019) and the increase in BACE levels (345% vs 123%, F = 42.24; P = 0.0002) induced by isoflurane. 2-DG decreased the levels of cytosolic calcium and ROS (100% vs 66%, F = 1.94; P = 0.014).Conclusions:These results suggest that 2-DG may decrease oxidative stress and increase cytosolic calcium levels, thus attenuating isoflurane-induced neurotoxicity. |
| | |
Authors:
|
Jun Zhang; Yuanlin Dong; Zhipeng Xu; Yiying Zhang; Chuxiong Pan; Sayre McAuliffe; Fumito Ichinose; Yun Yue; Weimin Liang; Zhongcong Xie |
Related Documents
:
|
17275357 - Heat shock protein gp96 is a master chaperone for toll-like receptors and is important ... 18202507 - Injury to allografts: innate immune pathways to acute and chronic rejection. 16428797 - Induction of in vivo antipolysaccharide immunoglobulin responses to intact streptococcu... 19010387 - Testicular innate immune defense against bacteria. 6604737 - Effect of midbrain stimulus-induced analgesia on immune function in humans. 20406797 - New insights into paracrine mechanisms of human cardiac progenitor cells. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-9-29 |
Journal Detail:
|
Title: Anesthesia and analgesia Volume: - ISSN: 1526-7598 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
|
Created Date: 2011-10-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 1310650 Medline TA: Anesth Analg Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
From the *Geriatric Anesthesia Research Unit. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Automated Machine Checkout Leaves an Internal Gas Leak Undetected: The Need for Complete Checkout Pr...
Next Document: Enhancing the Relative Safety of Intentional or Unintentional Intrathecal Methylprednisolone Adminis...