Document Detail


2-DE and MS analysis of interactions between Lactobacillus fermentum I5007 and intestinal epithelial cells.
MedLine Citation:
PMID:  18004711     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lactobacillus is a probiotic commonly used for supplementation to human and animal diets. In this study, we used 2-DE and MS to analyze changes in the proteomes of Lactobacillus and intestinal epithelial cells in two model systems. The in vivo and in vitro models were involved the inoculation of Lactobacillus fermentum I5007 into the rabbit jejunum for 4 h and the culture of the bacterium with Caco-2 cells for 1 h, respectively. Our results indicate that, after exposure to the intestinal environment, the bacterium exhibited decreases in key enzymes involved in energy metabolism (e.g., lactate dehydrogenase, dihydrolipoamide dehydrogenase, and nicotinate phosphoribosyltransferase) and amino acid metabolism (e.g., arginyl-tRNA synthetase and aspartate-semialdehyde dehydrogenase), but increases in glycoside hydrolase (an enzyme for mucin degradation) and fructose-6-phosphate phosphoketolase (an enzyme of the pentose phosphate pathway). In response to an interaction with L. fermentum I5007, Caco-2 cells showed changes in proteins that were beneficial for gut integrity, including voltage-dependent anion channel 1, glutathione transferase, and heat shock protein gp96. On the basis of their functions, we suggest that these proteins serve as useful biomarkers for metabolic changes in Lactobacillus and intestinal epithelial cells in response to their interactions.
Authors:
Fang Yang; Junjun Wang; Xiaojie Li; Tianyi Ying; Shiyan Qiao; Defa Li; Guoyao Wu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  28     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  4330-9     Citation Subset:  IM    
Affiliation:
National Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Neoplasm / analysis,  metabolism
Bacterial Adhesion
Biological Markers / analysis
Caco-2 Cells / metabolism
Cell Communication*
Cell Extracts / analysis
Coculture Techniques / methods
Electrophoresis, Gel, Two-Dimensional / methods*
Energy Metabolism
Glutathione Transferase / analysis,  metabolism
Humans
Intestinal Mucosa / chemistry,  metabolism,  microbiology*
Jejunum / cytology,  microbiology
Lactobacillus fermentum / chemistry,  metabolism*
Neoplasm Proteins / analysis
Probiotics / pharmacology
Proteome / analysis
Rabbits
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Voltage-Dependent Anion Channel 1 / analysis,  metabolism
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Biological Markers; 0/Cell Extracts; 0/Neoplasm Proteins; 0/Proteome; 0/sarcoma glycoprotein gp96 rejection antigens; EC 1.6.-/Voltage-Dependent Anion Channel 1; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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